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Literature summary extracted from

  • Tafesse, F.G.; Huitema, K.; Hermansson, M.; van der Poel, S.; van den Dikkenberg, J.; Uphoff, A.; Somerharju, P.; Holthuis, J.C.
    Both sphingomyelin synthases SMS1 and SMS2 are required for sphingomyelin homeostasis and growth in human HeLa cells (2007), J. Biol. Chem., 282, 17537-17547.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.27 expression analysis of isozymes SMS1 and SMS2 by RT-PCR Mus musculus
2.7.8.27 expression analysis of isozymes SMS1 and SMS2 by RT-PCR, recombinant overexpression of isozymes SMS1 and SMS2 in HeLa cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.27 additional information endogenous SMS2 expression in HeLa cells is reduced by about 80% after treatment with SMS2 siRNA, but is unaffected after treatment with lamin A or SMS1 siRNAs. Impact of SMS1 and SMS2 depletion on the lipid composition of HeLa cells, overview Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.27 Golgi membrane
-
 Mus musculus 139
-
2.7.8.27 Golgi membrane isozyme SMS1 Homo sapiens 139
-
2.7.8.27 additional information immunohistochemical localization study Homo sapiens
-
-
2.7.8.27 perinuclear space a small portion of SMS2 is found in the perinuclear region Homo sapiens
-
-
2.7.8.27 plasma membrane
-
 Mus musculus 5886
-
2.7.8.27 plasma membrane isozyme SMS2 Homo sapiens 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.27 Mg2+
-
 Homo sapiens
2.7.8.27 Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.27 additional information Homo sapiens both sphingomyelin synthases SMS1 and SMS2 are required for sphingomyelin homeostasis and growth in human HeLa cells, sphingomyelin synthesis plays a critical role in cell growth and survival, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.27 Homo sapiens
-
 isozymes SMS1 and SMS2
-
2.7.8.27 Mus musculus
-
 isozymes SMS1 and SMS2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.27 Caco-2 cell isozymes SMS1 and SMS2 Homo sapiens
-
2.7.8.27 fibroblast isozymes SMS1 and SMS2 Mus musculus
-
2.7.8.27 HeLa cell SMS1 represents a major whereas SMS2 represents a minor SM-synthase activity in HeLa cells Homo sapiens
-
2.7.8.27 Hep-G2 cell isozymes SMS1 and SMS2 Homo sapiens
-
2.7.8.27 MEB-4 cell isozymes SMS1 and SMS2 Mus musculus
-
2.7.8.27 melanoma cell isozymes SMS1 and SMS2 Mus musculus
-
2.7.8.27 myofibroblast cell line isozymes SMS1 and SMS2 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.27 additional information both sphingomyelin synthases SMS1 and SMS2 are required for sphingomyelin homeostasis and growth in human HeLa cells, sphingomyelin synthesis plays a critical role in cell growth and survival, overview Homo sapiens ?
-
 ?
2.7.8.27 phosphatidylcholine + C6-NBD-ceramide i.e. N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-erythro-sphingosine, the fluorogenic derivative of C6-ceramide Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.27 SM synthase
-
 Mus musculus
2.7.8.27 SM synthase
-
 Homo sapiens
2.7.8.27 SMS1
-
 Mus musculus
2.7.8.27 SMS1
-
 Homo sapiens
2.7.8.27 SMS2
-
 Mus musculus
2.7.8.27 SMS2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.27 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.27 7.4
-
 assay at Homo sapiens