Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Moe, E.; Leiros, I.; Smalas, A.O.; McSweeney, S.
    The crystal structure of mismatch-specific uracil-DNA glycosylase (MUG) from Deinococcus radiodurans reveals a novel catalytic residue and broad substrate specificity (2006), J. Biol. Chem., 281, 569-577.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 MUG, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of wild-type and mutant enzymes in Escherichia coli strain Bl21(DE3) Deinococcus radiodurans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.27 purified recombinant wild-type MUG and MUG mutant D93A, crystal growth by mixing 0.001 ml drops of 10 mg/ml protein with a solution containing 0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate, pH 6.5, and 30% w/v polyethylene glycol 8000, equilibration at 18°C, hexagonal crystals suitable for data collection purposes appear after 1 day, X-ray diffraction structure determination and analysis at 1.7-1.75 A resolution, molecular replacement Deinococcus radiodurans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.27 D93A site-directed mutagenesis, inactive mutant Deinococcus radiodurans

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Deinococcus radiodurans Q9RWH9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.27 recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) Deinococcus radiodurans

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.2.27 Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil catalytic reaction mechanism, the catalytic residue is Asp93 Deinococcus radiodurans
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.27 0.01
-
 purified recombinant MUG Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 uracil-mismatched double-stranded DNA + H2O 30-bp dsDNA oligonucleotide substrates containing G-C, G-U, G-T, and A-U base pairs. Determination of kinetics using commercially available nick-translated calf thymus DNA with deoxy[5-3H]uridine 5'-triphosphate as substrate. To perform efficient glycoside bond cleavage, drMUG must stabilize the mismatched uracil in the specificity pocket, nucleotide stabilization by Tyr46, substrate binding mechanism, overview. Binding of thymine in the activity pocket is probably prevented by Ser36 and Ser39 in MUG, binding of cytosine is prevented by Asp84 Deinococcus radiodurans uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched single-stranded DNA + H2O single-stranded DNA containing uracil labeled with fluorescein in the 5'-end, sequence overview. Determination of kinetics using commercially available nick-translated calf thymus DNA with deoxy[5-3H]uridine 5'-triphosphate as substrate. To perform efficient glycoside bond cleavage, drMUG must stabilize the mismatched uracil in the specificity pocket, nucleotide stabilization by Tyr46, substrate binding mechanism, overview. Binding of thymine in the activity pocket is probably prevented by Ser36 and Ser39 in MUG, binding of cytosine is prevented by Asp84 Deinococcus radiodurans uracil + single-stranded DNA with abasic site
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 mismatch-specific uracil-DNA glycosylase
-
 Deinococcus radiodurans
3.2.2.27 More three MUGs form a distintc MUG/thymidine-DNA glycosylase subfamily, called the MUG2 family Deinococcus radiodurans
3.2.2.27 MUG
-
 Deinococcus radiodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.27 37
-
 assay at Deinococcus radiodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.27 8
-
 assay at Deinococcus radiodurans