Any feedback?
Literature summary extracted from
- Saccharomyces cerevisiae MPH1 gene, required for homologous recombination-mediated mutation avoidance, encodes a 3' to 5' DNA helicase (2005), J. Biol. Chem., 280, 7854-7860.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.4 | heterotrimeric single-stranded DNA binding protein replication protein A | enhances DNA helicase activity of Mph1 | Saccharomyces cerevisiae | |
| 5.6.2.4 | single-stranded DNA | the enzyme requires single-stranded DNA for activation | Saccharomyces cerevisiae |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.6.2.4 | the carboxyl-terminal His6 epitope is attached to the MPH1-coding sequence and the tagged gene is placed under the galactose inducible GAL1 promoter in the vector pYES | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.4 | KCl | 30% inhibition occurs when KCl concentration is increased from 15 to 200 mM | Saccharomyces cerevisiae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.4 | Mg2+ | maximal activity is obtained with Mg2+, whereas Co2+ and Ca2+ are much less effective in this regard. No activity is observed with Mn2+ or Zn2+ | Saccharomyces cerevisiae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.2.4 | Saccharomyces cerevisiae | P40562 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.6.2.4 | - |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.4 | ATP + H2O | DNA helicase activity has a 3' to 5' polarity with respect to the DNA strand on which this protein translocates | Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.6.2.4 | 3' to 5' DNA helicase | - |
Saccharomyces cerevisiae |
| 5.6.2.4 | MPH1 | - |
Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.4 | 6.6 | 8.4 | - |
Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
