EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.115 | gene flaA1, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), complementation of a Pseudomonas aeruginosa WbpM knockout by expression of His-tagged FlaA1 | Helicobacter pylori |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.115 | soluble | - |
Helicobacter pylori | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.115 | 37400 | - |
2 * 37400, recombinant His-tagged enzyme, SDS-PAGE | Helicobacter pylori |
4.2.1.115 | 75000 | - |
about, recombinant His-tagged enzyme, gel filtration | Helicobacter pylori |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.115 | UDP-N-acetylglucosamine | Helicobacter pylori | - |
UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.115 | Helicobacter pylori | - |
strains 26695 and J99 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.115 | recombinant His-tagged FlaA1 9.9fold from Escherichia coli strain BL21(DE3) to homogeneity by nickel chelation and cation exchange chromatography | Helicobacter pylori |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.115 | additional information | FlaA1 is a bifunctional C6 dehydratase/C4 reductase specific for UDPGlcNAc. It converts UDP-GlcNAc into a UDP-4-keto-6-methyl-GlcNAc intermediate, which is stereospecifically reduced into UDP-QuiNAc | Helicobacter pylori | ? | - |
? | |
4.2.1.115 | UDP-N-acetylglucosamine | - |
Helicobacter pylori | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.115 | dimer | 2 * 37400, recombinant His-tagged enzyme, SDS-PAGE | Helicobacter pylori |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.115 | FlaA1 | - |
Helicobacter pylori |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.115 | 37 | - |
assay at | Helicobacter pylori |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.115 | 15 | 65 | - |
Helicobacter pylori |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.115 | 7 | - |
assay at | Helicobacter pylori |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.115 | NAD+ | - |
Helicobacter pylori |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.2.1.115 | Helicobacter pylori | sequence calculation | - |
8.65 |