EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.2.2 | expressed in Escherichia coli BL21(DE3) cells | Sphingobium chungbukense |
5.4.2.8 | expressed in Escherichia coli BL21(DE3) cells | Sphingobium chungbukense |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.2.2 | 0.2228 | - |
alpha-D-glucose 1-phosphate | SP-1, in 50 mM Tris-HCl (pH 7.5), at 37°C | Sphingobium chungbukense | |
5.4.2.2 | 0.4392 | - |
alpha-D-mannose 1-phosphate | SP-1, in 50 mM Tris-HCl (pH 7.5), at 37°C | Sphingobium chungbukense | |
5.4.2.8 | 0.1569 | - |
alpha-D-mannose 1-phosphate | in 50 mM Tris-HCl (pH 7.5), at 37°C | Sphingobium chungbukense | |
5.4.2.8 | 0.4516 | - |
alpha-D-glucose 1-phosphate | in 50 mM Tris-HCl (pH 7.5), at 37°C | Sphingobium chungbukense |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.4.2.2 | additional information | PGM activity of SP-1 requires divalent metal ions | Sphingobium chungbukense | |
5.4.2.8 | Mg2+ | highest activity in the presence of Mg2+ | Sphingobium chungbukense |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.4.2.2 | 57500 | - |
deduced from amino acid sequence | Sphingobium chungbukense |
5.4.2.2 | 58000 | - |
SDS-PAGE | Sphingobium chungbukense |
5.4.2.2 | 59500 | - |
native enzyme, non-denaturing PAGE | Sphingobium chungbukense |
5.4.2.8 | 51000 | - |
2 * 51000, SDS-PAGE | Sphingobium chungbukense |
5.4.2.8 | 105400 | - |
native enzyme, non-denaturing PAGE | Sphingobium chungbukense |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.2.2 | Sphingobium chungbukense | A8D008 | fragment | - |
5.4.2.2 | Sphingobium chungbukense DJ77 | A8D008 | fragment | - |
5.4.2.8 | Sphingobium chungbukense | - |
- |
- |
5.4.2.8 | Sphingobium chungbukense DJ77 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.2.2 | Ni-chelating resin chromatography | Sphingobium chungbukense |
5.4.2.8 | nickel-chelating resin chromatography | Sphingobium chungbukense |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.2.2 | alpha-D-glucose 1-phosphate | SP-1 shows preference for glucose-1-phosphate rather than mannose-1-phosphate | Sphingobium chungbukense | alpha-D-glucose 6-phosphate | - |
? | |
5.4.2.2 | alpha-D-glucose 1-phosphate | SP-1 shows preference for glucose-1-phosphate rather than mannose-1-phosphate | Sphingobium chungbukense DJ77 | alpha-D-glucose 6-phosphate | - |
? | |
5.4.2.2 | alpha-D-mannose 1-phosphate | SP-1 shows preference for glucose-1-phosphate rather than mannose-1-phosphate | Sphingobium chungbukense | D-mannose-6-phosphate | - |
? | |
5.4.2.2 | alpha-D-mannose 1-phosphate | SP-1 shows preference for glucose-1-phosphate rather than mannose-1-phosphate | Sphingobium chungbukense DJ77 | D-mannose-6-phosphate | - |
? | |
5.4.2.8 | alpha-D-glucose 1-phosphate | - |
Sphingobium chungbukense | alpha-D-glucose 6-phosphate | - |
? | |
5.4.2.8 | alpha-D-glucose 1-phosphate | - |
Sphingobium chungbukense DJ77 | alpha-D-glucose 6-phosphate | - |
? | |
5.4.2.8 | alpha-D-mannose 1-phosphate | preferred substrate | Sphingobium chungbukense | alpha-D-mannose 6-phosphate | - |
? | |
5.4.2.8 | alpha-D-mannose 1-phosphate | preferred substrate | Sphingobium chungbukense DJ77 | alpha-D-mannose 6-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.4.2.2 | monomer | 1 * 59500, native enzyme, non-denaturing PAGE | Sphingobium chungbukense |
5.4.2.8 | homodimer | 2 * 51000, SDS-PAGE | Sphingobium chungbukense |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.2.2 | PGM | - |
Sphingobium chungbukense |
5.4.2.2 | SP-1 | bifunctional enzyme with phosphoglucomutase and phosphomannomutase activities | Sphingobium chungbukense |
5.4.2.8 | PGM/PMM | - |
Sphingobium chungbukense |
5.4.2.8 | SP-2 | bifunctional enzyme with phosphoglucomutase and phosphomannomutase activities | Sphingobium chungbukense |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.2.2 | 79.17 | - |
alpha-D-mannose 1-phosphate | SP-1, in 50 mM Tris-HCl (pH 7.5), at 37°C | Sphingobium chungbukense | |
5.4.2.2 | 126.8 | - |
alpha-D-glucose 1-phosphate | SP-1, in 50 mM Tris-HCl (pH 7.5), at 37°C | Sphingobium chungbukense | |
5.4.2.8 | 82.93 | - |
alpha-D-glucose 1-phosphate | in 50 mM Tris-HCl (pH 7.5), at 37°C | Sphingobium chungbukense | |
5.4.2.8 | 134.9 | - |
alpha-D-mannose 1-phosphate | in 50 mM Tris-HCl (pH 7.5), at 37°C | Sphingobium chungbukense |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.2.2 | 7.4 | - |
- |
Sphingobium chungbukense |
5.4.2.8 | 7.5 | - |
- |
Sphingobium chungbukense |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.4.2.2 | 7 | 9 | - |
Sphingobium chungbukense |
5.4.2.8 | 7 | 9 | - |
Sphingobium chungbukense |