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Literature summary extracted from

  • Scaramozzino, F.; White, A.; Perego, M.; Hoch, J.A.
    A unique GTP dependent sporulation sensor histidine kinase in Bacillus anthracis (2008), J. Bacteriol., 191, 687-692.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.13.3 additional information the virulence plasmid-encoded sensor domains of BA2291 have a regulatory effect in vivo on the activity of full-length BA2291, by converting it from a normally functional sporulation kinase to an enzyme that inhibits sporulation, molecular mechanism Bacillus anthracis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.13.3 gene BA2291, genes for orthologs of the sensor domain of the BA2291 kinase exist in virulence plasmids in this organism, and these proteins, when expressed, inhibit sporulation by converting BA2291 to an apparent phosphatase of the sporulation phosphorelay. Expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus anthracis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.13.3 additional information the BA2291 gene is capable of complementing Bacillus subtilis sporulation kinase-deficient mutants when present in the cell in single copy, and its deletion in Bacillus anthracis strongly reduces the ability of the organism to sporulate. BA2291 overexpression in Bacillus subtilis completely prevents sporulation, also in a wild type strain Bacillus anthracis
2.7.13.3 S147L a BA2291 mutant, which mimics the behavior of the overexpressed wild-type protein.The S147L mutation does not affect the interaction of the kinase with Spo0F or the rate of phosphoryl group transfer to it Bacillus anthracis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.13.3 additional information the virulence plasmid-encoded sensor domains of BA2291 have a regulatory effect in vivo on the activity of full-length BA2291, by converting it from a normally functional sporulation kinase to an enzyme that inhibits sporulation, molecular mechanism Bacillus anthracis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.13.3 44000
-
 2 * 44000, SDS-PAGE Bacillus anthracis
2.7.13.3 88000
-
 about, gel filtration Bacillus anthracis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.13.3 GTP + protein-L-histidine Bacillus anthracis the enzyme is involved the transition between the vegetative cycle and sporulation, multiple sensor histidine kinases are induced to autophosphorylate in response to sporulation specific signals in the phosphorelay, the BA2291 protein may act as a phosphatase on the sporulation phosphorelay when present at elevated levels, overview GDP + protein N-phospho-L-histidine
-
 r
2.7.13.3 GTP + protein-L-histidine Bacillus anthracis 34F2DELTA118 the enzyme is involved the transition between the vegetative cycle and sporulation, multiple sensor histidine kinases are induced to autophosphorylate in response to sporulation specific signals in the phosphorelay, the BA2291 protein may act as a phosphatase on the sporulation phosphorelay when present at elevated levels, overview GDP + protein N-phospho-L-histidine
-
 r
2.7.13.3 GTP + Spo0F L-histidine Bacillus anthracis a protein purified from Bacillus subtilis GDP + Spo0F N-phospho-L-histidine
-
 r
2.7.13.3 GTP + Spo0F L-histidine Bacillus anthracis 34F2DELTA118 a protein purified from Bacillus subtilis GDP + Spo0F N-phospho-L-histidine
-
 r
2.7.13.3 additional information Bacillus anthracis genes for orthologs of the sensor domain of the BA2291 kinase exist in virulence plasmids in this organism, and these proteins, when expressed, inhibit sporulation by converting BA2291 to an apparent phosphatase of the sporulation phosphorelay ?
-
 ?
2.7.13.3 additional information Bacillus anthracis 34F2DELTA118 genes for orthologs of the sensor domain of the BA2291 kinase exist in virulence plasmids in this organism, and these proteins, when expressed, inhibit sporulation by converting BA2291 to an apparent phosphatase of the sporulation phosphorelay ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.13.3 Bacillus anthracis A0A2P0HDR3 gene BA2291, the organism contains nine genes encoding putative sporulation histidine kinases
-
2.7.13.3 Bacillus anthracis 34F2DELTA118 A0A2P0HDR3 gene BA2291, the organism contains nine genes encoding putative sporulation histidine kinases
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.13.3 phosphoprotein BA2291 performs GTP-dependent autophosphorylation Bacillus anthracis

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.13.3 recombinant His6-tagged BA2291 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Bacillus anthracis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.13.3 GTP + protein-L-histidine the enzyme is involved the transition between the vegetative cycle and sporulation, multiple sensor histidine kinases are induced to autophosphorylate in response to sporulation specific signals in the phosphorelay, the BA2291 protein may act as a phosphatase on the sporulation phosphorelay when present at elevated levels, overview Bacillus anthracis GDP + protein N-phospho-L-histidine
-
 r
2.7.13.3 GTP + protein-L-histidine BA2291 is absolutely specific for guanine nucleotides in both the forward and reverse reactions, nucleotide specificity of BA2291, overview Bacillus anthracis GDP + protein N-phospho-L-histidine
-
 r
2.7.13.3 GTP + protein-L-histidine the enzyme is involved the transition between the vegetative cycle and sporulation, multiple sensor histidine kinases are induced to autophosphorylate in response to sporulation specific signals in the phosphorelay, the BA2291 protein may act as a phosphatase on the sporulation phosphorelay when present at elevated levels, overview Bacillus anthracis 34F2DELTA118 GDP + protein N-phospho-L-histidine
-
 r
2.7.13.3 GTP + protein-L-histidine BA2291 is absolutely specific for guanine nucleotides in both the forward and reverse reactions, nucleotide specificity of BA2291, overview Bacillus anthracis 34F2DELTA118 GDP + protein N-phospho-L-histidine
-
 r
2.7.13.3 GTP + Spo0F L-histidine a protein purified from Bacillus subtilis Bacillus anthracis GDP + Spo0F N-phospho-L-histidine
-
 r
2.7.13.3 GTP + Spo0F L-histidine a protein purified from Bacillus subtilis Bacillus anthracis 34F2DELTA118 GDP + Spo0F N-phospho-L-histidine
-
 r
2.7.13.3 additional information genes for orthologs of the sensor domain of the BA2291 kinase exist in virulence plasmids in this organism, and these proteins, when expressed, inhibit sporulation by converting BA2291 to an apparent phosphatase of the sporulation phosphorelay Bacillus anthracis ?
-
 ?
2.7.13.3 additional information BA2291 performs GTP-dependent autophosphorylation Bacillus anthracis ?
-
 ?
2.7.13.3 additional information genes for orthologs of the sensor domain of the BA2291 kinase exist in virulence plasmids in this organism, and these proteins, when expressed, inhibit sporulation by converting BA2291 to an apparent phosphatase of the sporulation phosphorelay Bacillus anthracis 34F2DELTA118 ?
-
 ?
2.7.13.3 additional information BA2291 performs GTP-dependent autophosphorylation Bacillus anthracis 34F2DELTA118 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.13.3 dimer 2 * 44000, SDS-PAGE Bacillus anthracis

Synonyms

EC Number Synonyms Comment Organism
2.7.13.3 BA2291
-
 Bacillus anthracis
2.7.13.3 sensor histidine kinase
-
 Bacillus anthracis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.13.3 GDP this kinase is uniquely specific for GTP in the forward and GDP in the reverse reaction. The G1 motif of BA2291 is highly modified from ATP specific histidine kinases, binding structure, modeling, overview Bacillus anthracis
2.7.13.3 GTP this kinase is uniquely specific for GTP in the forward and GDP in the reverse reaction. The G1 motif of BA2291 is highly modified from ATP specific histidine kinases, binding structure, modeling, overview Bacillus anthracis
2.7.13.3 additional information no activity with ATP, TTP, or CTP in autophosphorylation Bacillus anthracis