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Literature summary extracted from

  • Higgs, P.I.; Jagadeesan, S.; Mann, P.; Zusman, D.R.
    EspA, an orphan hybrid histidine protein kinase, regulates the timing of expression of key developmental proteins of Myxococcus xanthus (2008), J. Bacteriol., 190, 4416-4426.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.13.3 expresssion of GST-tagged wild-type and mutant enzymes, quantitative expression analysis, overview Myxococcus xanthus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.13.3 D696A site-directed mutagenesis, the mutation of the receiver domain residue leads to release of phosphate to the medium, overview Myxococcus xanthus
2.7.13.3 H407A site-directed mutagenesis, the mutation of the autophosphorylation site residue H407 inhibits autophosphorylation of the enzyme Myxococcus xanthus
2.7.13.3 additional information construction of several mutants by in-frame deletions, espA mutants, encoding an orphan hybrid histidine kinase, alter the timing of the organism's developmental program, greatly accelerating developmental progression, phenotype, overview Myxococcus xanthus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.13.3 cytosol
-
 Myxococcus xanthus 5829
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Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.13.3 83000
-
 x * 83000, SDS-PAGE Myxococcus xanthus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.13.3 additional information Myxococcus xanthus Myxococcus xanthus undergoes a complex starvation-induced developmental program that results in cells forming multicellular fruiting bodies by aggregating into mounds and then differentiating into spores. This developmental program involves EspA, which plays a key role in the timing of expression of genes necessary for progression of cells through the developmental program, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.13.3 Myxococcus xanthus Q9S434 wild-type strain DZ2, gene espA
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Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.13.3 phosphoprotein the enzyme autophosphorylates in vitro on the conserved histidine residue and then transfers the phosphoryl group to the conserved aspartate residue in the associated receiver domain Myxococcus xanthus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.13.3 additional information EspA developmental expression pattern, mutational analysis, overview Myxococcus xanthus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.13.3 additional information Myxococcus xanthus undergoes a complex starvation-induced developmental program that results in cells forming multicellular fruiting bodies by aggregating into mounds and then differentiating into spores. This developmental program involves EspA, which plays a key role in the timing of expression of genes necessary for progression of cells through the developmental program, overview Myxococcus xanthus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.13.3 ? x * 83000, SDS-PAGE Myxococcus xanthus

Synonyms

EC Number Synonyms Comment Organism
2.7.13.3 EspA
-
 Myxococcus xanthus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.13.3 22
-
 assay at room temperature Myxococcus xanthus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.13.3 6.8
-
 assay at Myxococcus xanthus