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Literature summary extracted from

  • Fuhrer, T.; Chen, L.; Sauer, U.; Vitkup, D.
    Computational prediction and experimental verification of the gene encoding the NAD+/NADP+-dependent succinate semialdehyde dehydrogenase in Escherichia coli (2007), J. Bacteriol., 189, 8073-8078.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.16 gamma-aminobutyrate induces GabD dehydrogenase Escherichia coli
1.2.1.24 additional information gene yneI is responsible for NAD+/NADP+-SSADH activity. The gene is induced by succinate semialdehyde Escherichia coli

Application

EC Number Application Comment Organism
1.2.1.16 additional information the NADP+-dependent succinate semialdehyde dehydrogenase activity encoded by gabD appears to be important for nitrogen metabolism under N limitation conditions Escherichia coli
1.2.1.24 additional information yneI, responsible for NAD+/NADP+-dependent SSADH activity, plays a unique physiological role in the general nitrogen metabolism of Escherichia coli. The yneI gene has an important, but not essential, role during growth on arginine and probably has an essential function during growth on putrescine as the nitrogen source. The yneI-encoded activity functions primarily as a valve to prevent toxic accumulation of succinate semialdehyde Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.16 overexpressed as His-tagged GabD Escherichia coli
1.2.1.24 overexpressed as His-tagged YneI Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.16 additional information gabD knockout mutant has a limited capacity to metabolize gamma-aminobutyrate Escherichia coli
1.2.1.24 additional information yneI knockout mutant shows strongly inhibited growth compared to the wild-type Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.24 50000
-
 YneI protein Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Escherichia coli
-
-
-
1.2.1.16 Escherichia coli BW25113
-
-
-
1.2.1.24 Escherichia coli
-
-
-
1.2.1.24 Escherichia coli BW25113
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.16 His-tagged GabD dehydrogenase purified from cell extracts with HiTrap affinity columns Escherichia coli
1.2.1.24 His-tagged YneI dehydrogenase purified from cell extracts with HiTrap affinity columns Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Escherichia coli succinate + NADPH + H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Escherichia coli BW25113 succinate + NADPH + H+
-
 ?
1.2.1.24 succinate semialdehyde + NAD+ + H2O
-
 Escherichia coli succinate + NADH + H+
-
 ?
1.2.1.24 succinate semialdehyde + NAD+ + H2O
-
 Escherichia coli BW25113 succinate + NADH + H+
-
 ?
1.2.1.24 succinate semialdehyde + NADP+ + H2O
-
 Escherichia coli succinate + NADPH + H+
-
 ?
1.2.1.24 succinate semialdehyde + NADP+ + H2O
-
 Escherichia coli BW25113 succinate + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.16 GabD dehydrogenase
-
 Escherichia coli
1.2.1.16 SSADH
-
 Escherichia coli
1.2.1.16 succinate semialdehyde dehydrogenase
-
 Escherichia coli
1.2.1.24 SSADH
-
 Escherichia coli
1.2.1.24 succinate semialdehyde dehydrogenase
-
 Escherichia coli
1.2.1.24 YneI dehydrogenase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NADP+ GabD dehydrogenase is specific for NADP+ Escherichia coli
1.2.1.24 NAD+
-
 Escherichia coli
1.2.1.24 NADP+
-
 Escherichia coli