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Literature summary extracted from
- Cloning and characterization of an antifungal class III chitinase from suspension-cultured bamboo (Bambusa oldhamii) cells (2008), J. Agric. Food Chem., 56, 11507-11514.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | gene Chi3-1, expression in Pichia pastoris strain X-33 | Bambusa oldhamii |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 28300 | - |
x * 35700, glycosylated Chi3, SDS-PAGE, x * 28300, nonglycosylated Chi3, SDS-PAGE | Bambusa oldhamii |
| 3.2.1.14 | 35700 | - |
x * 35700, glycosylated Chi3, SDS-PAGE, x * 28300, nonglycosylated Chi3, SDS-PAGE | Bambusa oldhamii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | chitin + H2O | Bambusa oldhamii | - |
? | - |
? |  |
| 3.2.1.14 | additional information | Bambusa oldhamii | recombinant glycosylated and nonglycosylated BoCHI3-1 isoforms show antifungal activity against Scolecobasidium longiphorum | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Bambusa oldhamii | - |
gene Chi3-1 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | glycoprotein | - |
Bambusa oldhamii |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | recombinant Chi3 from Pichia pastoris strain X-33 by sequential ammonium sulfate fractionation, hydrophobic interaction chromatography, and Con A affinity chromatography to homogeneity | Bambusa oldhamii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 117 | - |
purified recombinant nonglycosylated Chi3 | Bambusa oldhamii |
| 3.2.1.14 | 289 | - |
purified recombinant glycosylated Chi3 | Bambusa oldhamii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | chitin + H2O | - |
Bambusa oldhamii | ? | - |
? | |
| 3.2.1.14 | chitin + H2O | cleavage product analysis, overview | Bambusa oldhamii | ? | - |
? | |
| 3.2.1.14 | ethylene glycol chitin + H2O | - |
Bambusa oldhamii | ? | - |
? | |
| 3.2.1.14 | additional information | recombinant glycosylated and nonglycosylated BoCHI3-1 isoforms show antifungal activity against Scolecobasidium longiphorum | Bambusa oldhamii | ? | - |
? | |
| 3.2.1.14 | additional information | Chi3 acts as an endohydrolase, it cleaves chitin trimers into monomers and dimers and cleaves chitin tetramer to hexamer into a mixture of monomers, dimers, and trimers | Bambusa oldhamii | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | ? | x * 35700, glycosylated Chi3, SDS-PAGE, x * 28300, nonglycosylated Chi3, SDS-PAGE | Bambusa oldhamii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | class III chitinase | - |
Bambusa oldhamii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 70 | - |
nonglycosylated enzyme Chi3 | Bambusa oldhamii |
| 3.2.1.14 | 80 | - |
glycosylated enzyme Chi3 | Bambusa oldhamii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 3 | - |
glycosylated enzyme Chi3 | Bambusa oldhamii |
| 3.2.1.14 | 4 | - |
nonglycosylated enzyme Chi3 | Bambusa oldhamii |
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