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Literature summary extracted from

  • Perrin, B.; Rowland, M.; Wolfe, M.; Tsigelny, I.; Pezzementi, L.
    Thermal denaturation of wild type and mutant recombinant acetylcholinesterase from amphioxus: effects of the temperature of in vitro expression and of reversible inhibitors (2008), Invert. Neurosci., 8, 147-155.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.7 expression of wild-type and mutant enzymes in COS-7 cells Branchiostoma floridae

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.7 C310A site-directed mutagenesis, the mutant shows altered thermal stability and sensitivity to inhibitors compared to the wild-type enzyme Branchiostoma floridae
3.1.1.7 C310A/C466A site-directed mutagenesis, the mutant shows altered thermal stability and sensitivity to inhibitors compared to the wild-type enzyme Branchiostoma floridae
3.1.1.7 C310A/F312I site-directed mutagenesis, the mutant shows unaltered thermal stability but altered sensitivity to inhibitors compared to the wild-type enzyme Branchiostoma floridae
3.1.1.7 C466A site-directed mutagenesis, the mutant shows altered thermal stability and sensitivity to inhibitors compared to the wild-type enzyme Branchiostoma floridae
3.1.1.7 F312I site-directed mutagenesis, the mutant shows altered thermal stability and sensitivity to inhibitors compared to the wild-type enzyme Branchiostoma floridae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 BW284c51
-
 Branchiostoma floridae
3.1.1.7 edrophonium
-
 Branchiostoma floridae
3.1.1.7 ethopropazine
-
 Branchiostoma floridae
3.1.1.7 propidium
-
 Branchiostoma floridae

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Branchiostoma floridae
-
-
-

Subunits

EC Number Subunits Comment Organism
3.1.1.7 More structure homology molecular modelling, overview Branchiostoma floridae

Synonyms

EC Number Synonyms Comment Organism
3.1.1.7 ChE2
-
 Branchiostoma floridae
3.1.1.7 cholinesterase 2
-
 Branchiostoma floridae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.7 30 37 assay at Branchiostoma floridae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.7 30 37 thermal inactivation rates and kinetics of wild-type and mutant enzymes, overview Branchiostoma floridae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.7 7
-
 assay at Branchiostoma floridae