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Literature summary extracted from
- Role of trehalose-6P phosphatase (TPS2) in stress tolerance and resistance to macrophage killing in Candida albicans (2009), Int. J. Med. Microbiol., 299, 453-464.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.12 | expressed in Escherichia coli | Candida albicans |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.12 | trehalose 6-phosphate + H2O | Candida albicans | pleiotropic defective phenotype of disrupted TPS2, cell wall integrity and ability to form chlamydospores maintained, reduced growth and temperature sensitivity (42°C) of homozygous mutant, severe oxidative exposure (50 mM H2O2), null mutants reveal adaptive antioxidant response and cross-tolerance between temperature and oxidative stress, expression of TPS2 and TPS1 genes promoted in wild-type cells in response to acute (50 mM) but not gentle (5 mM) oxidative exposure | trehalose + phosphate | - |
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Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.15 | Candida albicans | - |
- |
- |
| 3.1.3.12 | Candida albicans | Q5AI14 | wild-type SC5314 and mutant | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.12 | gel filtration | Candida albicans |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.12 | additional information | - |
pleiotropic defective phenotype of disrupted TPS2, cell wall integrity and ability to form chlamydospores maintained, reduced growth and temperature sensitivity (42°C) of homozygous mutant, severe oxidative exposure (50 mM H2O2), null mutants reveal adaptive antioxidant response and cross-tolerance between temperature and oxidative stress, differential measurement of trehalose and trehalose 6-phosphate by HPLC, accumulation of trehalose 6-phosphate in null mutants but not in parental cells, accumulation of trehalose 6-phosphate enhanced after oxidative exposure, expression of TPS2 and TPS1 genes promoted in wild-type cells in response to acute (50 mM) but not gentle (5 mM) oxidative exposure, transcriptional response absent in mutants, co-culture of yeast cells with macrophages and fungicidal assays, role of trehalose biosynthetic pathway in the cellular response to oxidative stress | Candida albicans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.12 | trehalose 6-phosphate + H2O | pleiotropic defective phenotype of disrupted TPS2, cell wall integrity and ability to form chlamydospores maintained, reduced growth and temperature sensitivity (42°C) of homozygous mutant, severe oxidative exposure (50 mM H2O2), null mutants reveal adaptive antioxidant response and cross-tolerance between temperature and oxidative stress, expression of TPS2 and TPS1 genes promoted in wild-type cells in response to acute (50 mM) but not gentle (5 mM) oxidative exposure | Candida albicans | trehalose + phosphate | - |
? | |
| 3.1.3.12 | trehalose 6-phosphate + H2O | protective role of the trehalose biosynthetic pathway in the cellular response to oxidative stress and subsequently in the resistance to phagocytosis | Candida albicans | trehalose + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.12 | TPS2 | - |
Candida albicans |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 2.4.1.15 | Candida albicans | a transitory increase in the expression of TPS2 and TPS1 genes is promoted in wild-type cells inresponse to acute, but not to gentleoxidative exposure | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.4.1.15 | physiological function | disruption of the TPS2 gene encoding the only trehalose 6-phosphate phosphatase activity in Candida albicans causes a pleiotropic defective phenotype, maintaining the cell wall integrity and the ability to form chlamydospores. A homozygous tps2Delta/tps2Delta shows reduced growth at high temperatures and a marked sensitivity to heatshock and severe oxidative exposure. Exponential tps2Delta null cells display an adaptive response to oxidative stress as well as cross-tolerance between temperature and oxidative stress. Differential measurement of trehalose and trehalose 6-phosphate, revealed a significant accumulation of trehalose 6-phosphate in tps2Delta cells, which is enhanced after oxidative exposure. The level of trehalose 6-phosphate in parental cells is virtually undetectable, and oxidative treatment only induces the synthesis of free trehalose | Candida albicans |
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