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Literature summary extracted from

  • Carvalho, A.B.; de Assis, S.A.; Leite, K.M.; Bach, E.E.; Oliveira, O.M.
    Pectin methylesterase activity and ascorbic acid content from guava fruit, cv. Predilecta, in different phases of development (2008), Int. J. Food Sci. Nutr., 60, 255-265.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.11 additional information PME activity increases with fruit maturation Psidium guajava
3.1.1.11 NaCl activity is highest in the presence of 0.3 M NaCl in 50 mM borate-acetate, pH 8.3 Psidium guajava

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.11 57000
-
 guava PME contains two isoforms, one with 57000 Da molecular mass, SDS-PAGE Psidium guajava
3.1.1.11 99000
-
 guava PME contains two isoforms, one with 99000 Da molecular mass, SDS-PAGE Psidium guajava

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Psidium guajava
-
 cultivar Predilecta
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11 ammonium sulfate fractionation Psidium guajava

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.11 fruit PME activity increases with fruit maturation Psidium guajava
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 pectin + H2O citrus pectin Psidium guajava methanol + pectate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.11 pectin methylesterase
-
 Psidium guajava
3.1.1.11 PME
-
 Psidium guajava

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.11 95
-
-
 Psidium guajava

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.11 50 98 guava PME retains 96.8% of activity after 300 min in 90°C, retains 101.85% of its specific activity after 60 min of incubation at 50°C, the PME enzyme retains 75.4%, 86.2% and 90.4% of its specific activity after 60 min of incubation, respectively, at 80°C, 90°C, and 98°C Psidium guajava

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.11 10
-
-
 Psidium guajava