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Literature summary extracted from
- A yeast PAF acetylhydrolase ortholog suppresses oxidative death (2008), Free Radic. Biol. Med., 45, 434-442.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.47 | into pYES-DEST52 using the pENTR/D-TOPO cloning kit, expression in Saccharomyces cerevisiae cells | Schizosaccharomyces pombe |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.1.47 | S257A | serine 257 is predicted to be the active site serine | Schizosaccharomyces pombe |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.47 | 55000 | - |
determined by SDS-PAGE and Western Blot analysis | Schizosaccharomyces pombe |
| 3.1.1.47 | 55000 | - |
predicted | Schizosaccharomyces pombe |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.47 | Schizosaccharomyces pombe | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.47 | 0.0000005 | - |
mutant S257A, substrate azelaoyl phosphatidylcholine | Schizosaccharomyces pombe |
| 3.1.1.47 | 0.00001 | - |
wild type, substrate azelaoyl phosphatidylcholine | Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.47 | azelaoyl phosphatidylcholine + H2O | - |
Schizosaccharomyces pombe | ? | - |
? |  |
| 3.1.1.47 | platelet-activating factor + H2O | - |
Schizosaccharomyces pombe | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.47 | group VII PAF acetylhydrolase homolog | - |
Schizosaccharomyces pombe |
| 3.1.1.47 | PAF acetylhydrolase | ortholog | Schizosaccharomyces pombe |
| 3.1.1.47 | platelet-activating factor acetylhydrolase | - |
Schizosaccharomyces pombe |
| 3.1.1.47 | Plg7p | - |
Schizosaccharomyces pombe |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.47 | 37 | - |
activity assay | Schizosaccharomyces pombe |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.47 | 7.8 | - |
activity assay | Schizosaccharomyces pombe |
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Release 2023.1 (January 2023)
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