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Literature summary extracted from
- The dihydrolipoamide dehydrogenase from the crenarchaeon Acidianus ambivalens (2008), FEMS Microbiol. Lett., 281, 147-154.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.8.1.4 | additional information | metabolic context(s) of DLDHs remains an open question | Acidianus ambivalens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.4 | NADH | strong substrate inhibition at concentrations higher than 0.015 mM | Acidianus ambivalens |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.4 | 0.00315 | - |
NADH | - |
Acidianus ambivalens | |
| 1.8.1.4 | 0.7 | - |
dihydrolipoamide | - |
Acidianus ambivalens | |
| 1.8.1.4 | 0.71 | - |
NAD+ | - |
Acidianus ambivalens | |
| 1.8.1.4 | 1.26 | - |
Lipoamide | - |
Acidianus ambivalens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.4 | 45000 | - |
2 * 45400, sequence analysis, 2 * 45000, SDS-PAGE | Acidianus ambivalens |
| 1.8.1.4 | 45400 | - |
2 * 45400, sequence analysis, 2 * 45000, SDS-PAGE | Acidianus ambivalens |
| 1.8.1.4 | 107000 | - |
blue native PAGE | Acidianus ambivalens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.4 | Acidianus ambivalens | A4V929 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.4 | 0.36 | - |
purified DLDH | Acidianus ambivalens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.4 | dihydrolipoamide + NAD+ | DLDH activity, forward reaction | Acidianus ambivalens | lipoamide + NADH | - |
r | |
| 1.8.1.4 | lipoamide + NADH | NADH:lipoamide oxidoreductase activity, reverse reaction | Acidianus ambivalens | dihydrolipoamide + NAD+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.4 | homodimer | 2 * 45400, sequence analysis, 2 * 45000, SDS-PAGE | Acidianus ambivalens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.4 | dihydrolipoamide dehydrogenase | - |
Acidianus ambivalens |
| 1.8.1.4 | DLDH | - |
Acidianus ambivalens |
| 1.8.1.4 | NADH:lipoamide oxidoreductase | - |
Acidianus ambivalens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.4 | 55 | - |
NADH:lipoamide oxidoreductase activity | Acidianus ambivalens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.4 | 7 | - |
NADH:lipoamide oxidoreductase activity | Acidianus ambivalens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.4 | FAD | - |
Acidianus ambivalens | |
| 1.8.1.4 | NAD+ | NAD+ is prefered | Acidianus ambivalens | |
| 1.8.1.4 | NADH | - |
Acidianus ambivalens | |
| 1.8.1.4 | NADP+ | - |
Acidianus ambivalens | |
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