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Literature summary extracted from

  • McSorley, T.; Ort, S.; Hazra, S.; Lavie, A.; Konrad, M.
    Mimicking phosphorylation of Ser-74 on human deoxycytidine kinase selectively increases catalytic activity for dC and dC analogues (2008), FEBS Lett., 582, 720-724.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.74 expression in Escherichia coli and in HEK-293 cell Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.74 S74A 6-8fold lower activity than wild-type Homo sapiens
2.7.1.74 S74D 2-4fold lower kcat values Homo sapiens
2.7.1.74 S74E mutation mimicking phosphorylation, results in 11fold increase in kcat value for deoxycytidine. Mutation has no effect on cellular localization Homo sapiens
2.7.1.74 S74Q no significant change in kcat for any of the substrates tested Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 0.0017
-
 2'-deoxycytidine wild-type, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.0059
-
 1-beta-D-arabinofuranosylcytosine wild-type, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.0062
-
 2',2'-difluorodeoxycytidine wild-type, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.0089
-
 2'-deoxycytidine mutant S74E, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.015
-
 2',2'-difluorodeoxycytidine mutant S74E, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.024
-
 1-beta-D-arabinofuranosylcytosine mutant S74E, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.1169
-
 2'-deoxyadenosine wild-type, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.2458
-
 2'-deoxyguanosine wild-type, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.518
-
 2'-deoxyadenosine mutant S74E, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.602
-
 2'-deoxyguanosine mutant S74E, pH 7.5, 25°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.74 nucleus main localization of both wild-type and mutant S74E mimicking phosphorylated enzyme Homo sapiens 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.74 30000
-
 x * 30000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens P27707
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.74 phosphoprotein phosphorylation at residue S74 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 1-beta-D-arabinofuranosylcytosine
-
 Homo sapiens ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2',2'-difluorodeoxycytidine
-
 Homo sapiens ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxy-CMP
-
 ?
2.7.1.74 ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.74 ? x * 30000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.74 0.04
-
 2'-deoxycytidine wild-type, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.04
-
 2'-deoxycytidine mutant S74A, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.04
-
 2'-deoxycytidine mutant S74Q, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.16
-
 2'-deoxycytidine mutant S74D, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.3
-
 1-beta-D-arabinofuranosylcytosine mutant S74Q, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.32
-
 1-beta-D-arabinofuranosylcytosine wild-type, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.33
-
 1-beta-D-arabinofuranosylcytosine mutant S74A, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.36
-
 2'-deoxyguanosine mutant S74E, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.44
-
 2',2'-difluorodeoxycytidine wild-type, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.45
-
 2'-deoxycytidine mutant S74E, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.45
-
 2',2'-difluorodeoxycytidine mutant S74Q, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.5
-
 2',2'-difluorodeoxycytidine mutant S74A, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.57
-
 2'-deoxyguanosine mutant S74D, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.57
-
 1-beta-D-arabinofuranosylcytosine mutant S74D, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.65
-
 2'-deoxyguanosine mutant S74Q, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.68
-
 2'-deoxyguanosine mutant S74A, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.69
-
 2'-deoxyadenosine mutant S74E, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.73
-
 2'-deoxyguanosine wild-type, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.77
-
 2'-deoxyadenosine wild-type, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.9
-
 2',2'-difluorodeoxycytidine mutant S74D, pH 7.5, 25°C Homo sapiens
2.7.1.74 0.9
-
 2'-deoxyadenosine mutant S74Q, pH 7.5, 25°C Homo sapiens
2.7.1.74 1
-
 2'-deoxyadenosine mutant S74D, pH 7.5, 25°C Homo sapiens
2.7.1.74 1.06
-
 1-beta-D-arabinofuranosylcytosine mutant S74E, pH 7.5, 25°C Homo sapiens
2.7.1.74 1.12
-
 2'-deoxyadenosine mutant S74A, pH 7.5, 25°C Homo sapiens
2.7.1.74 1.21
-
 2',2'-difluorodeoxycytidine mutant S74E, pH 7.5, 25°C Homo sapiens