EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.231 | guanidine | no effect on wild-type but guanidine causes a 45fold enhancement of mutant R507A activity | Schizophyllum commune | |
2.4.1.231 | propargylamine | no effect on wild-type but propagylamine causes a 23fold enhancement of mutant K512A activity | Schizophyllum commune |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.231 | K512A | kcat/Km (phosphate): 0.0014/Msec, vanadate shows a weaker inhibitory effect compared to wild-type, propargylamine leads to a 23fold increase in activity, phosphate 50 mM enhances basal hydrolase activity | Schizophyllum commune |
2.4.1.231 | R507A | Km (phosphate) 103 mM, kcat/Km (phosphate): 0.0004/Msec, vanadate shows no inhibitory effect, guanidine leads to a 45fold increase in activity | Schizophyllum commune |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.231 | phosphate | phosphate 50 mM causes total supression in wild-type but enhances mutant K512 activity | Schizophyllum commune | |
2.4.1.231 | vanadate | strong inhibitor for wildtype ScTPase and weak inhibitor for mutant K512A but no mutant R507A is not inhibited at all | Schizophyllum commune |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.231 | additional information | - |
additional information | mutant K512A (phosphate): no saturation, Kcat/Km (phosphate): 0.0014/Msec | Schizophyllum commune | |
2.4.1.231 | 0.8 | - |
phosphate | wild-type, Kcat/Km (phosphate): 21000/Msec | Schizophyllum commune | |
2.4.1.231 | 103 | - |
phosphate | mutant R507A, Kcat/Km (phosphate): 0.0004/Msec | Schizophyllum commune |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.231 | Schizophyllum commune | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.231 | alpha,alpha-trehalose + phosphate | - |
Schizophyllum commune | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.231 | ScTPase | - |
Schizophyllum commune |
2.4.1.231 | trehalose phosphorylase | - |
Schizophyllum commune |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.231 | 30 | - |
assay at | Schizophyllum commune |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.231 | 6.6 | - |
assay at | Schizophyllum commune |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.231 | 0.0004 | - |
vanadate | wild-type | Schizophyllum commune | |
2.4.1.231 | 0.298 | - |
vanadate | mutant K512A | Schizophyllum commune |