EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.26 | alpha2-gliadin 33-mer peptide | POP inhibitor | Sus scrofa | |
3.4.21.26 | JTP-4819 | - |
Sus scrofa | |
3.4.21.26 | additional information | specific dipeptidyl peptidase IV inhibitor HIV-1 tat (1-9) fragment with sequence H-Met-Asp-Pro-Val-Asp-Pro-Asn-Ile-Glu-OH | Sus scrofa | |
3.4.21.26 | N-carbobenzoxy-Leu-Leu-Leu-COH | the proteasome inhibitor | Sus scrofa | |
3.4.21.26 | N-carbobenzoxy-prolyl-prolinal | ZPP | Sus scrofa | |
3.4.21.26 | phenylmethanesulfonyl fluoride | PE and POP are partially resistant to phenylmethanesulfonyl fluoride, a generic serine protease inhibitor that has a low efficiency in inhibiting POP | Sus scrofa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.21.26 | cytoplasm | - |
Sus scrofa | 5737 | - |
3.4.21.26 | extracellular | - |
Sus scrofa | - |
- |
3.4.21.26 | membrane | - |
Sus scrofa | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.26 | Cu2+ | - |
Sus scrofa | |
3.4.21.26 | Mn2+ | - |
Sus scrofa | |
3.4.21.26 | additional information | no significant differences between membrane PE and POP regarding the sensitivity to the metals | Sus scrofa | |
3.4.21.26 | NaCl | - |
Sus scrofa | |
3.4.21.26 | Ni2+ | - |
Sus scrofa | |
3.4.21.26 | Zn2+ | - |
Sus scrofa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.26 | 65000 | - |
higher salt concentrations used, membrane PE activity elutes with a molecular mass of 65 kDa | Sus scrofa |
3.4.21.26 | 80000 | - |
theoretical | Sus scrofa |
3.4.21.26 | 95000 | - |
membrane PE, conditions: 20 mm potassium phosphate, gel filtration | Sus scrofa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.26 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.26 | - |
Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.21.26 | brain | - |
Sus scrofa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.21.26 | 0.00017 | - |
high-density membranes, membrane-bound POP activity of different-density membrane fractions obtained by sucrose gradient centrifugation | Sus scrofa |
3.4.21.26 | 0.0002 | - |
washed membranes, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity | Sus scrofa |
3.4.21.26 | 0.00045 | - |
medium-density membranes, membrane-bound POP activity of different-density membrane fractions obtained by sucrose gradient centrifugation | Sus scrofa |
3.4.21.26 | 0.0005 | - |
total membranes, purification of mPOP from a total membrane preparation | Sus scrofa |
3.4.21.26 | 0.0005 | - |
washed membranes, membrane-bound POP activity of different-density membrane fractions obtained by sucrose gradient centrifugation | Sus scrofa |
3.4.21.26 | 0.00061 | - |
low-density membranes, membrane-bound POP activity of different-density membrane fractions obtained by sucrose gradient centrifugation | Sus scrofa |
3.4.21.26 | 0.001 | - |
crude extract, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity | Sus scrofa |
3.4.21.26 | 0.0014 | - |
unwashed membranes, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity | Sus scrofa |
3.4.21.26 | 0.0033 | - |
Triton X-100 extraction, purification of mPOP from a total membrane preparation | Sus scrofa |
3.4.21.26 | 0.0048 | - |
4 M NaCl wash, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity | Sus scrofa |
3.4.21.26 | 0.0074 | - |
DEAE, purification of mPOP from a total membrane preparation | Sus scrofa |
3.4.21.26 | 0.009 | - |
0.5 M NaCl wash, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity | Sus scrofa |
3.4.21.26 | 0.0138 | - |
water wash, POP activity partitioning during membrane preparation crude extract and membrane wash effects on recovered activity | Sus scrofa |
3.4.21.26 | 0.039 | - |
phenyl-sepharose, purification of mPOP from a total membrane preparation | Sus scrofa |
3.4.21.26 | 0.169 | - |
hydroxylapatite, purification of mPOP from a total membrane preparation | Sus scrofa |
3.4.21.26 | 0.857 | - |
second DEAE, purification of mPOP from a total membrane preparation | Sus scrofa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.26 | Ac-CDPGYIGSR-NH2 + H2O | substrate specificity studies on membrane PE as compared with POP. ZPP-sensitive cleavage of both occurred | Sus scrofa | ? | - |
? | |
3.4.21.26 | H-MDPVDPNIE-OH + H2O | substrate specificity studies on membrane PE as compared with POP. ZPP-sensitive cleavage of both occurred | Sus scrofa | ? | - |
? | |
3.4.21.26 | H2N-QLQPFPQPQLPY-OH + H2O | substrate specificity studies on membrane PE as compared with POP. ZPP-sensitive cleavage of both occurred | Sus scrofa | ? | - |
? | |
3.4.21.26 | additional information | substance P, arginine-vasopressin, thyroliberin and gonadoliberin are proposed physiological substrates of this protease. No ZPP-sensitive cleavage occurred with the 33-mer, PEP-3, PEP-26, PEP-48_2, and PEP-50 | Sus scrofa | ? | - |
? | |
3.4.21.26 | N-carbobenzoxy-Gly-Pro-7-amido-4-methyl-coumarin + H2O | - |
Sus scrofa | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.26 | membrane-bound PE | - |
Sus scrofa |
3.4.21.26 | mPOP | - |
Sus scrofa |
3.4.21.26 | POP | - |
Sus scrofa |
3.4.21.26 | prolyl endopeptidase | - |
Sus scrofa |