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Literature summary extracted from

  • Falaleeva, M.V.; Chetverina, H.V.; Ugarov, V.I.; Uzlova, E.A.; Chetverin, A.B.
    Factors influencing RNA degradation by Thermus thermophilus polynucleotide phosphorylase (2008), FEBS J., 275, 2214-2226.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.8 synthesis at the optimal temperature, polynucleotide phosphorylase completely destroys RNAs that possess even a very stable intramolecular secondary structure, but leaves intact RNAs whose 3' end is protected by chemical modification or by hybridization with a complementary oligonucleotide. This allows individual RNAs to be isolated from heterogeneous populations by degrading unprotected species. If oligonucleotide is hybridized to an internal RNA segment, the Tth polynucelotide phosphorylase stalls eight nucleotides downstream of that segment. This allows any arbitrary 5'-terminal fragment of RNA to be prepared with a precision similar to that of run-off transcription, but without the need for a restriction site Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.8 expression in Escherichia coli Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.8 Mg2+ required, enzyme retains significant activity when the concentration is in the micromolar range Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Thermus thermophilus Q9ZAE1
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 additional information at the optimal temperature, polynucleotide phosphorylase completely destroys RNAs that possess even a very stable intramolecular secondary structure, but leaves intact RNAs whose 3' end is protected by chemical modification or by hybridization with a complementary oligonucleotide. This allows individual RNAs to be isolated from heterogeneous populations by degrading unprotected species. If oligonucleotide is hybridized to an internal RNA segment, the Tth polynucelotide phosphorylase stalls eight nucleotides downstream of that segment. This allows any arbitrary 5'-terminal fragment of RNA to be prepared with a precision similar to that of run-off transcription, but without the need for a restriction site Thermus thermophilus ?
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 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.8 65
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-
 Thermus thermophilus