EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.3 | expressed in Escherichia coli DH5alpha cells | Pseudoalteromonas haloplanktis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | 2-mercaptoethanol | 43% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
3.1.1.3 | Ca2+ | 52% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
3.1.1.3 | Co2+ | 36% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
3.1.1.3 | Cu2+ | 57% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
3.1.1.3 | EDTA | 47% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
3.1.1.3 | Fe3+ | complete inhibition at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
3.1.1.3 | Hg2+ | 11% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
3.1.1.3 | Mg2+ | 54% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
3.1.1.3 | Mn2+ | 55% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
3.1.1.3 | phenylmethylsulfonyl fluoride | complete inhibition at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis | |
3.1.1.3 | SDS | 0.9% residual activity at 10 mM, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.3 | membrane | the enzyme is associated to the outer membrane | Pseudoalteromonas haloplanktis | 16020 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Pseudoalteromonas haloplanktis | Q3IF07 | - |
- |
3.1.1.3 | Pseudoalteromonas haloplanktis TAC 125 | Q3IF07 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.3 | ammonium sulfate precipitation and Q-Sepharose column chromatography | Pseudoalteromonas haloplanktis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 0.079 | - |
enzyme from solubilised medium, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis |
3.1.1.3 | 0.95 | - |
after 12fold purification, with 4-nitrophenyl caproate as substrate, at 25°C | Pseudoalteromonas haloplanktis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | 4-nitrophenyl acetate + H2O | - |
Pseudoalteromonas haloplanktis | 4-nitrophenol + acetate | - |
? | |
3.1.1.3 | 4-nitrophenyl acetate + H2O | - |
Pseudoalteromonas haloplanktis TAC 125 | 4-nitrophenol + acetate | - |
? | |
3.1.1.3 | 4-nitrophenyl caproate + H2O | best substrate | Pseudoalteromonas haloplanktis | 4-nitrophenol + caproate | - |
? | |
3.1.1.3 | 4-nitrophenyl hexanoate + H2O | - |
Pseudoalteromonas haloplanktis | 4-nitrophenol + hexanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl hexanoate + H2O | - |
Pseudoalteromonas haloplanktis TAC 125 | 4-nitrophenol + hexanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl octanoate + H2O | - |
Pseudoalteromonas haloplanktis | 4-nitrophenol + octanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl pentanoate + H2O | - |
Pseudoalteromonas haloplanktis | 4-nitrophenol + pentanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl pentanoate + H2O | - |
Pseudoalteromonas haloplanktis TAC 125 | 4-nitrophenol + pentanoate | - |
? | |
3.1.1.3 | tricaprylin + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
3.1.1.3 | tricaprylin + H2O | - |
Pseudoalteromonas haloplanktis TAC 125 | ? | - |
? | |
3.1.1.3 | triolein + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
3.1.1.3 | triolein + H2O | - |
Pseudoalteromonas haloplanktis TAC 125 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | Lip1 | - |
Pseudoalteromonas haloplanktis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 40 | - |
at pH 7.1 | Pseudoalteromonas haloplanktis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 5 | 50 | at pH 7.1 | Pseudoalteromonas haloplanktis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 5 | 40 | activity increases up to 40°C | Pseudoalteromonas haloplanktis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 8.5 | - |
reaches the apparent maximal activity at pH values above 8.5 | Pseudoalteromonas haloplanktis |