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Literature summary extracted from

  • Jin, C.M.; Yang, Y.J.; Huang, H.S.; Lim, S.C.; Kai, M.; Lee, M.K.
    Induction of dopamine biosynthesis by l-DOPA in PC12 cells: implications of L-DOPA influx and cyclic AMP (2008), Eur. J. Pharmacol., 591, 88-95.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.2 L-Dopa L-DOPA treatment (20-200 microM) increases the levels of dopamine by 226%-504% after 3-6 h of treatment and enhances the activities of tyrosine hydroxylase and aromatic L-amino acid decarboxylase Rattus norvegicus
4.1.1.28 L-Dopa L-DOPA treatment (20-200 microM) increases the levels of dopamine by 226%-504% after 3-6 h of treatment and enhances the activities of tyrosine hydroxylase and aromatic L-amino acid decarboxylase Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.2 L-Dopa Increased levels of intracellular L-DOPA inhibits tyrosine hydroxylase activity by end-product inhibition at early time points, L-DOPA (20-200 microM) treatment leads to a 562%-937% increase in L-DOPA influx at 1 h, which inhibits the activity of tyrosine hydroylase during the same period. Rattus norvegicus
4.1.1.28 Benserazide levels of intracellular dopamine after L-DOPA treatment (0.02 and 0.1 mM) are significantly decreased by the AADC inhibitor benserazide (0.02 mM) for 6-24 h exposure prior to L-DOPA treatment in PC12 cells, the 230%-350% increases in dopamine levels by L-DOPA are reduced to 187%-284% by benserazide for 6 h Rattus norvegicus
4.1.1.28 carbidopa levels of intracellular dopamine after L-DOPA treatment (0.02 and 0.1 mM) are significantly decreased by the AADC inhibitor carbidopa (0.020 mM) for 6-24 h exposure prior to L-DOPA treatment in PC12 cells, the 230%-350% increases in dopamine levels by L-DOPA are reduced to 187%-284% by 153%-248% by carbidopa for 6 h Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydropteridine + O2 Rattus norvegicus
-
 3,4-dihydroxyphenylalanine + dihydropteridine + H2O
-
 ?
4.1.1.28 3,4-Dihydroxyphenylalanine Rattus norvegicus L-DOPA, substrate exerts apoptotic cytotoxicity towards PC12 cells at a concentration of 0.1-0.2 mM for 24-48 h Dopamine + CO2 major amine neurotransmitter ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Rattus norvegicus
-
-
-
4.1.1.28 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.2 PC-12 cell derived from a pheochromocytoma of the rat adrenal medulla Rattus norvegicus
-
4.1.1.28 PC-12 cell derived from a pheochromocytoma of the rat adrenal medulla Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.2 additional information
-
 217% activity compared to control level, treatment with 0.02 mM L-DOPA in PC-12 cells at 6 h time point Rattus norvegicus
1.14.16.2 additional information
-
 232% activity compared to control level, treatment with 0.1 mM L-DOPA in PC-12 cells at 6 h time point Rattus norvegicus
1.14.16.2 additional information
-
 272% activity compared to control level, treatment with 0.02 mM L-DOPA in PC-12 cells at 6 h time point Rattus norvegicus
1.14.16.2 additional information
-
 55.2% activity compared to control level, treatment with 0.2 mM L-DOPA in PC-12 cells at 48 h time point Rattus norvegicus
1.14.16.2 additional information
-
 62.6% activity compared to control level, treatment with 0.2 mM L-DOPA in PC-12 cells at 1 h time point Rattus norvegicus
1.14.16.2 additional information
-
 68.2% activity compared to control level, treatment with 0.1 mM L-DOPA in PC-12 cells at 1 h time point Rattus norvegicus
1.14.16.2 additional information
-
 82.2% activity of tyrosine hydroxylase compared to control level, treatment with 0.02 mM L-DOPA in PC-12 cells at 1 h time point Rattus norvegicus
1.14.16.2 additional information
-
 82.3% activity compared to control level, treatment with 0.1 mM L-DOPA in PC-12 cells at 48 h time point Rattus norvegicus
1.14.16.2 additional information
-
 activity returns to the control level between 24-48 h after treatment with 0.02 mM L-DOPA. Rattus norvegicus
1.14.16.2 0.00368
-
 activity of tyrosine hydroxylase, control level without treatment of L-DOPA in PC-12 cells Rattus norvegicus
4.1.1.28 additional information
-
 110% activity compared to control level, treatment with 0.02 mM L-DOPA in PC-12 cells at 1 h (time point) Rattus norvegicus
4.1.1.28 additional information
-
 237% activity compared to control level, treatment with 0.02 mM L-DOPA in PC-12 cells at 3 h (time point) Rattus norvegicus
4.1.1.28 additional information
-
 242% activity compared to control level, treatment with 0.1 mM L-DOPA in PC-12 cells at 1 h (time point) Rattus norvegicus
4.1.1.28 additional information
-
 269% activity compared to control level, treatment with f 0.1 mM L-DOPA in PC-12 cells at 3 h (time point) Rattus norvegicus
4.1.1.28 additional information
-
 311% activity compared to control level, treatment with 0.2 mM L-DOPA in PC-12 cells at 1 h (time point) Rattus norvegicus
4.1.1.28 additional information
-
 348% activity compared to control level, treatment with 0.2 mM L-DOPA in PC-12 cells at 3 h (time point) Rattus norvegicus
4.1.1.28 additional information
-
 The activity of AADC begins to decrease to about 177% (0.02 mM L-DOPA), 233% (0.1 mM L-DOPA) and 297% (0.2 mM L-DOPA) of the control levels by 3-6 h, and then returns to control level by about 24-48 h Rattus norvegicus
4.1.1.28 0.0325
-
 activity of AADC, control level without treatment of L-DOPA in PC-12 cells Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydropteridine + O2
-
 Rattus norvegicus 3,4-dihydroxyphenylalanine + dihydropteridine + H2O
-
 ?
4.1.1.28 3,4-Dihydroxyphenylalanine L-DOPA, substrate exerts apoptotic cytotoxicity towards PC12 cells at a concentration of 0.1-0.2 mM for 24-48 h Rattus norvegicus Dopamine + CO2 major amine neurotransmitter ?

Synonyms

EC Number Synonyms Comment Organism
1.14.16.2 tyrosine hydroxylase
-
 Rattus norvegicus
4.1.1.28 AADC
-
 Rattus norvegicus
4.1.1.28 Aromatic L-amino acid decarboxylase
-
 Rattus norvegicus