EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.2.27 | drug development | the UDG from Mycobacterium smegmatis might be a useful target for inhibitor design | Mycolicibacterium smegmatis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.27 | UGD inhibition protein from Bacillus subtilis | phage PBS-2-encoded uracil DNA glycosylase inhibitor, UDG forms a dissociable, activity-reduced complex with the inhibitor protein Ugi in 1:1 molar stoichiometry | Mycolicibacterium smegmatis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | 25000 | - |
x * 25000, SDS-PAGE | Mycolicibacterium smegmatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.27 | additional information | Mycolicibacterium smegmatis | UDG initiates uracil excision repair to safeguard the genomic integrity, mechanism, overview | ? | - |
? | |
3.2.2.27 | additional information | Mycolicibacterium smegmatis SN2 | UDG initiates uracil excision repair to safeguard the genomic integrity, mechanism, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.27 | Mycolicibacterium smegmatis | - |
- |
- |
3.2.2.27 | Mycolicibacterium smegmatis SN2 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.27 | native enzyme 3111fold by anion exchange chromatography, gel filtration, another step of a different anion exchange chromatography, and DNA affinity and hydroxyapatatite chromatography | Mycolicibacterium smegmatis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | 14000 | - |
purified native enzyme | Mycolicibacterium smegmatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.27 | additional information | UDG initiates uracil excision repair to safeguard the genomic integrity, mechanism, overview | Mycolicibacterium smegmatis | ? | - |
? | |
3.2.2.27 | additional information | Mycobacterium smegmatis UDG excises uracil from different loop positions of tetraloop hairpin substrates with comparable efficiencies, substrate Escherichia coli RZ1032 dut1 ung1 genomic DNA | Mycolicibacterium smegmatis | ? | - |
? | |
3.2.2.27 | additional information | UDG initiates uracil excision repair to safeguard the genomic integrity, mechanism, overview | Mycolicibacterium smegmatis SN2 | ? | - |
? | |
3.2.2.27 | additional information | Mycobacterium smegmatis UDG excises uracil from different loop positions of tetraloop hairpin substrates with comparable efficiencies, substrate Escherichia coli RZ1032 dut1 ung1 genomic DNA | Mycolicibacterium smegmatis SN2 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.2.27 | ? | x * 25000, SDS-PAGE | Mycolicibacterium smegmatis |
3.2.2.27 | More | the initiating amino acid, formyl-methionine is cleaved from the mature UDG protein | Mycolicibacterium smegmatis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.2.27 | uracil DNA glycosylase | - |
Mycolicibacterium smegmatis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | 37 | - |
assay at | Mycolicibacterium smegmatis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | 8 | - |
first optimum | Mycolicibacterium smegmatis |
3.2.2.27 | 8.5 | 9 | second optimum | Mycolicibacterium smegmatis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | 6.5 | 9.5 | - |
Mycolicibacterium smegmatis |