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Literature summary extracted from

  • Velloso, L.M.; Bhaskaran, S.S.; Schuch, R.; Fischetti, V.A.; Stebbins, C.E.
    A structural basis for the allosteric regulation of non-hydrolysing UDP-GlcNAc 2-epimerases. [Erratum to document cited in CA148:302197] (2008), EMBO Rep., 9, 1251.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.3.14 UDP-GlcNAc allosteric activitation by substrate via direct interactions between UDP and substrate Bacillus anthracis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.3.14 ternary complex between the UDP-GlcNAc 2-epimerase, its substrate UDP-GlcNAc and the reaction intermediate UDP, at 1.7 A resolution. Direct interactions between the substrate and UDP via two hydrogen bonds to the alpha- and beta-phosphates of the adjacent UDP molecule, and between the complex and highly conserved enzyme residues. The binding of UDP-GlcNAc is associated with conformational changes in the active site of the enzyme Bacillus anthracis

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.14 H242A dramatic decrease in catalytic efficiency Bacillus anthracis
5.1.3.14 H44Q dramatic decrease in catalytic efficiency Bacillus anthracis
5.1.3.14 Q43A dramatic decrease in catalytic efficiency Bacillus anthracis
5.1.3.14 Q70A dramatic decrease in catalytic efficiency Bacillus anthracis
5.1.3.14 R210A dramatic decrease in catalytic efficiency Bacillus anthracis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.14 2.2
-
 UDP-N-acetyl-D-glucosamine wild-type Bacillus anthracis
5.1.3.14 7.3
-
 UDP-N-acetyl-D-glucosamine mutant H44Q Bacillus anthracis
5.1.3.14 10.4
-
 UDP-N-acetyl-D-glucosamine mutant H242A Bacillus anthracis
5.1.3.14 11.1
-
 UDP-N-acetyl-D-glucosamine mutant Q70A Bacillus anthracis
5.1.3.14 17.2
-
 UDP-N-acetyl-D-glucosamine mutant Q43A Bacillus anthracis
5.1.3.14 20.9
-
 UDP-N-acetyl-D-glucosamine mutant R210A Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.14 Bacillus anthracis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.14 UDP-N-acetyl-D-glucosamine non-hydrolizing Bacillus anthracis UDP-N-acetyl-D-mannosamine
-
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Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.14 0.021
-
 UDP-N-acetyl-D-glucosamine mutant R210A Bacillus anthracis
5.1.3.14 0.022
-
 UDP-N-acetyl-D-glucosamine mutant H242A Bacillus anthracis
5.1.3.14 1.2
-
 UDP-N-acetyl-D-glucosamine mutant Q43A Bacillus anthracis
5.1.3.14 2.06
-
 UDP-N-acetyl-D-glucosamine mutant Q70A Bacillus anthracis
5.1.3.14 4
-
 UDP-N-acetyl-D-glucosamine mutant H44Q Bacillus anthracis
5.1.3.14 7.9
-
 UDP-N-acetyl-D-glucosamine wild-type Bacillus anthracis