Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yan, L.J.; Yang, S.H.; Shu, H.; Prokai, L.; Forster, M.J.
    Histochemical staining and quantification of dihydrolipoamide dehydrogenase diaphorase activity using blue native PAGE (2007), Electrophoresis, 28, 1036-1045.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.4 additional information activity of DLDH diaphorase is both protein amount- and time-dependent Rattus norvegicus

Application

EC Number Application Comment Organism
1.8.1.4 analysis development of a blue native-PAGE-based method for isolation of enzymatically active DLDH from animal tissues and visualization as well as quantification of its diaphorase activity using the NADH/nitroblue tetrazolium detection system Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.4 Angeli's salt at 2 mM, induces a 90% loss in DLDH diaphorase activity Rattus norvegicus
1.8.1.4 Diethylamine NONOate induces 71% loss in diaphorase activity at 10 mM, but does not induce any activity loss at 2 mM Rattus norvegicus
1.8.1.4 additional information diamide, GSH, GSSG, cysteine and nitrite do not exhibit any inhibitory effects Rattus norvegicus
1.8.1.4 N-ethylmaleimide induces more than 95% loss in DLDH diaphorase activity Rattus norvegicus
1.8.1.4 S-nitrosocysteine induces a 62% loss in diaphorase activity at 2 mM and an 88% loss at 10 mM Rattus norvegicus
1.8.1.4 S-nitrosoglutathione induces 84% loss in diaphorase activity at 10 mM, but does not induce any activity loss at 2 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.4 mitochondrion DLDH exclusively exists in mitochondria Rattus norvegicus 5739
-
1.8.1.4 additional information no DLDH diaphorase activity in the cytosol Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Rattus norvegicus
-
 adult Sprague-Dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4 gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.4 brain
-
 Rattus norvegicus
-
1.8.1.4 heart
-
 Rattus norvegicus
-
1.8.1.4 kidney
-
 Rattus norvegicus
-
1.8.1.4 liver
-
 Rattus norvegicus
-
1.8.1.4 lung
-
 Rattus norvegicus
-
1.8.1.4 skeletal muscle
-
 Rattus norvegicus
-
1.8.1.4 testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 nitro blue tetrazolium + NADH
-
 Rattus norvegicus ? + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 dihydrolipoamide dehydrogenase
-
 Rattus norvegicus
1.8.1.4 DLDH
-
 Rattus norvegicus
1.8.1.4 DLDH dehydrogenase
-
 Rattus norvegicus
1.8.1.4 DLDH diaphorase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 additional information no activity with NADPH Rattus norvegicus
1.8.1.4 NADH
-
 Rattus norvegicus