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Literature summary extracted from

  • Wu, H.; White, C.C.; Isanhart, J.P.; McBride, T.J.; Kavanagh, T.J.; Hooper, M.J.
    Optimization and application of glutamate cysteine ligase measurement in wildlife species (2008), Ecotoxicol. Environ. Saf., 72, 572-578.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.2 L-cysteine varying glutamic acid concentrations from 5 to 80 mM do not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influence GCL activities substantially in a tissue-dependent manner, about 20 mM L-Cys is optimal in the different tissue, overview. After subacute exposure, low doses increases GCL activity and GSH content in liver by 48.3% and 54.4%, respectively. High doses reduce GCL activities significantly in liver and kidney to 31.2% and 43.0% of the control, respectively Anas platyrhynchos
6.3.2.2 L-cysteine varying glutamic acid concentrations from 5 to 80 mM do not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influence GCL activities substantially in a tissue-dependent manner, about 20 mM L-Cys is optimal in the different tissue, overview. Low doses activate high doses inhibits the enzyme Mus musculus
6.3.2.2 L-cysteine varying glutamic acid concentrations from 5 to 80 mM do not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influence GCL activities substantially in a tissue-dependent manner, about 20 mM L-Cys is optimal in the different tissue, overview. Low doses activate high doses inhibits the enzyme Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 L-cysteine varying glutamic acid concentrations from 5 to 80 mM do not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influence GCL activities substantially in a tissue-dependent manner, about 20 mM L-Cys is optimal in the different tissue, overview. After subacute exposure, low doses increases GCL activity and GSH content in liver by 48.3% and 54.4%, respectively. High doses reduce GCL activities significantly in liver and kidney to 31.2% and 43.0% of the control, respectively Anas platyrhynchos
6.3.2.2 L-cysteine varying glutamic acid concentrations from 5 to 80 mM do not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influence GCL activities substantially in a tissue-dependent manner, about 20 mM L-Cys is optimal in the different tissue, overview. Low doses activate high doses inhibits the enzyme Mus musculus
6.3.2.2 L-cysteine varying glutamic acid concentrations from 5 to 80 mM do not affect GCL activities markedly, whereas cysteine concentrations from 2.5 to 40 mM influence GCL activities substantially in a tissue-dependent manner, about 20 mM L-Cys is optimal in the different tissue, overview. Low doses activate high doses inhibits the enzyme Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.2 Mg2+
-
 Mus musculus
6.3.2.2 Mg2+
-
 Rattus norvegicus
6.3.2.2 Mg2+
-
 Anas platyrhynchos

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.2 ATP + L-glutamate + L-cysteine Mus musculus rate-limiting enzyme in glutathione biosynthesis ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine Rattus norvegicus rate-limiting enzyme in glutathione biosynthesis ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine Anas platyrhynchos rate-limiting enzyme in glutathione biosynthesis ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Anas platyrhynchos
-
 mallard duck
-
6.3.2.2 Mus musculus
-
 deer mice
-
6.3.2.2 Rattus norvegicus
-
 Sprague-Dawley rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.2 brain
-
 Mus musculus
-
6.3.2.2 brain
-
 Rattus norvegicus
-
6.3.2.2 brain
-
 Anas platyrhynchos
-
6.3.2.2 heart
-
 Mus musculus
-
6.3.2.2 heart
-
 Rattus norvegicus
-
6.3.2.2 heart
-
 Anas platyrhynchos
-
6.3.2.2 kidney
-
 Mus musculus
-
6.3.2.2 kidney
-
 Rattus norvegicus
-
6.3.2.2 kidney
-
 Anas platyrhynchos
-
6.3.2.2 liver
-
 Mus musculus
-
6.3.2.2 liver
-
 Rattus norvegicus
-
6.3.2.2 liver
-
 Anas platyrhynchos
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.2 additional information
-
-
 Mus musculus
6.3.2.2 additional information
-
-
 Rattus norvegicus
6.3.2.2 additional information
-
-
 Anas platyrhynchos

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-glutamate + L-cysteine
-
 Mus musculus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine
-
 Rattus norvegicus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine
-
 Anas platyrhynchos ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine rate-limiting enzyme in glutathione biosynthesis Mus musculus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine rate-limiting enzyme in glutathione biosynthesis Rattus norvegicus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine rate-limiting enzyme in glutathione biosynthesis Anas platyrhynchos ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.2 GCL
-
 Mus musculus
6.3.2.2 GCL
-
 Rattus norvegicus
6.3.2.2 GCL
-
 Anas platyrhynchos
6.3.2.2 glutamate cysteine ligase
-
 Mus musculus
6.3.2.2 glutamate cysteine ligase
-
 Rattus norvegicus
6.3.2.2 glutamate cysteine ligase
-
 Anas platyrhynchos

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.2 ATP
-
 Mus musculus
6.3.2.2 ATP
-
 Rattus norvegicus
6.3.2.2 ATP
-
 Anas platyrhynchos