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Literature summary extracted from

  • Naidu, R.B.; Saisubramanian, N.; Selvakumar, D.; Janardhanan, S.; Puvanakrishnan, R.
    Partial purification of tannase from Aspergillus foetidus by aqueous two phase extraction and its characterization (2008), Curr. Trends Biotechnol. Pharm., 2, 201-207.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 Mg2+ slight reduction in activity in the presence of Mg2+ Aspergillus foetidus
3.1.1.20 Zn2+ slight reduction in activity in the presence of Zn2+ Aspergillus foetidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 Ca2+ highly stable in presence of 1 mM CaCl2 Aspergillus foetidus
3.1.1.20 Cu2+ highly stable in presence of 1 mM CuSO4 Aspergillus foetidus
3.1.1.20 Mn2+ highly stable in presence of 1 mM MnSO4 Aspergillus foetidus
3.1.1.20 Na+ highly stable in presence of 1 mM NaCl Aspergillus foetidus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.20 additional information the enzyme shows considerable stability in the presence of commercial detergents such as Sunlight, Rinshakthi and Wheel Aspergillus foetidus
3.1.1.20 SDS the enzyme shows considerable stability in the presence of SDS Aspergillus foetidus
3.1.1.20 Triton X-100 considerable loss in activity in the presence of Triton X-100 Aspergillus foetidus
3.1.1.20 Tween 60 considerable loss in activity in the presence of Tween 60 Aspergillus foetidus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus foetidus
-
-
-
3.1.1.20 Aspergillus foetidus MTCC 6322
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 partial purification by ammonium sulfate precipitation and aqueous two phase extraction with PEG6000 Aspergillus foetidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 33.7
-
 crude extract Aspergillus foetidus
3.1.1.20 88.3
-
 after 2.62fold purification Aspergillus foetidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 tannic acid + H2O
-
 Aspergillus foetidus gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus foetidus MTCC 6322 gallate + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acyl hydrolase
-
 Aspergillus foetidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 30
-
-
 Aspergillus foetidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.20 15 40
-
 Aspergillus foetidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 40
-
 significant decrease in thermal stability at temperatures above 40°C Aspergillus foetidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 6
-
-
 Aspergillus foetidus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.20 5 7
-
 Aspergillus foetidus