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Literature summary extracted from

  • Haushalter, K.A
    Stukenberg, P.T.; Kirschner, M.W.; Verdine, G.L.: Identification of a new uracil-DNA glycosylase family by expression cloning using synthetic inhibitors (1999), Curr. Biol., 9, 174-185.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 clone TD12, DNA and amino acid sequence determination and analysis, expression as C-terminally His6-tagged enzyme in Escherichia coli Xenopus laevis
3.2.2.27 DNA and amino acid sequence determination and analysis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.27 additional information design of mechanism-based inhibitors, overview Homo sapiens
3.2.2.27 additional information design of mechanism-based inhibitors, overview Xenopus laevis
3.2.2.27 UGI inhibitor
-
 Homo sapiens
3.2.2.27 UGI inhibitor
-
 Xenopus laevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.27 additional information
-
 additional information kinetics Xenopus laevis
3.2.2.27 0.000035
-
 uracil-mismatched double-stranded DNA with U-G mismatch
-
 Xenopus laevis
3.2.2.27 0.00109
-
 uracil-mismatched single-stranded DNA
-
 Xenopus laevis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.27 nucleus
-
 Xenopus laevis 5634
-
3.2.2.27 nucleus
-
 Homo sapiens 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.27 31000
-
 x * 31000, SMUG1, SDS-PAGE Xenopus laevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Xenopus laevis
-
 uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched single-stranded DNA + H2O Xenopus laevis
-
 uracil + single-stranded DNA with abasic site
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Homo sapiens Q53HV7
-
-
3.2.2.27 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.27 recombinant C-terminally His6-tagged enzyme from Escherichia coli by a 2-step procedure Xenopus laevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 additional information SMUG1 is a monofunctional DNA glycosylase specific for uracil residues, and has appreciable selectivity for single-stranded rather than double-stranded DNA substrates, overview Xenopus laevis ?
-
 ?
3.2.2.27 additional information SMUG1 is a monofunctional DNA glycosylase specific for uracil residues, and has appreciable selectivity for single-stranded rather than double-stranded DNA substrates, overview Homo sapiens ?
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O
-
 Xenopus laevis uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O
-
 Homo sapiens uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA with U-G mismatch + H2O
-
 Xenopus laevis uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched single-stranded DNA + H2O
-
 Xenopus laevis uracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched single-stranded DNA + H2O
-
 Homo sapiens uracil + single-stranded DNA with abasic site
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.27 ? x * 31000, SMUG1, SDS-PAGE Xenopus laevis

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 single-strand-selective monofunctional uracil-DNA glycosylase
-
 Xenopus laevis
3.2.2.27 single-strand-selective monofunctional uracil-DNA glycosylase
-
 Homo sapiens
3.2.2.27 SMUG1
-
 Xenopus laevis
3.2.2.27 SMUG1
-
 Homo sapiens
3.2.2.27 TD12-encoded protein
-
 Xenopus laevis
3.2.2.27 uracil-DNA glycosylase
-
 Xenopus laevis
3.2.2.27 uracil-DNA glycosylase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.27 0.0014
-
 uracil-mismatched double-stranded DNA with U-G mismatch
-
 Xenopus laevis
3.2.2.27 2.5
-
 uracil-mismatched single-stranded DNA
-
 Xenopus laevis