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Literature summary extracted from

  • Pandey, M.K.; Sung, B.; Kunnumakkara, A.B.; Sethi, G.; Chaturvedi, M.M.; Aggarwal, B.B.
    Berberine modifies cysteine 179 of IkappaBalpha kinase, suppresses nuclear factor-kappaB-regulated antiapoptotic gene products, and potentiates apoptosis (2008), Cancer Res., 68, 5370-5379.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.10 TNF-alpha
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.10 C179A berberine does not have any effect on mutant IKK beta C179A. This proves that berberine inhibits IKK beta activity through the modification of Cys179 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.10 Berberine an isoquinoline alkaloid derived from a plant used traditionally in Chinese and Ayurvedic medicine inhibits IKK activity. Addition of DTT to the kinase reaction reverses the berberine-mediated inhibition of IKK activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.10 Homo sapiens O14920
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.10 HEK-293 cell
-
 Homo sapiens
-
2.7.11.10 JURKAT cell
-
 Homo sapiens
-
2.7.11.10 lung adenocarcinoma cell line H1299 Homo sapiens
-
2.7.11.10 U-266 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.10 ATP + IkappaBalpha
-
 Homo sapiens ADP + phosphorylated IkappaBalpha
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.10 IkappaB alpha kinase
-
 Homo sapiens
2.7.11.10 IKK beta
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.10 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.10 7.4
-
 assay at Homo sapiens