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Literature summary extracted from

  • La, I.; Kalme, S.; Yoon, M.
    Reversible denaturation and reactivation of aspartase from Hafnia alvei (2008), Bull. Korean Chem. Soc., 29, 208-210.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.3.1.1 industry aspartate synthesis Hafnia alvei

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.1 guanidine hydrochloride at concentrations lower than 1 M, activity is gradually decreased suggesting the existence of the native tetrameric form with denaturation intermediates such as dimeric and monomeric forms of the enzyme. At higher concentrations above 1 M, the enzyme completely lost the activity, suggesting that the enzyme structure is completely denatured Hafnia alvei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.1 55000
-
-
 Hafnia alvei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.1 L-aspartate Hafnia alvei
-
 fumarate + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.1 Hafnia alvei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.1 by using a combination of diethylaminoethyl cellulose, Red A-agarose,and Sepharose 6B chromatography Hafnia alvei

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.3.1.1 100% activity recovery is observed when the enzyme is renatured by dilution at concentrations below 1 M guanidine hydrochloride, the renaturation yield at concentrations above 1 M is 40%. The dissociation process from native tetramer to dimer is reversible but the dissociation process from dimer to monomer is not reversible. Hafnia alvei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.1.1 cell culture
-
 Hafnia alvei
-

Storage Stability

EC Number Storage Stability Organism
4.3.1.1 the catalytic function of the purified enzyme which stored at -70°C until use remains stable for at least 1 month at 4°C without appreciable loss of enzymatic activity Hafnia alvei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.1 L-aspartate
-
 Hafnia alvei fumarate + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
4.3.1.1 homotetramer
-
 Hafnia alvei

Synonyms

EC Number Synonyms Comment Organism
4.3.1.1 aspartase
-
 Hafnia alvei
4.3.1.1 L-aspartate ammonia-lyase
-
 Hafnia alvei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.1 25
-
 optimum temperature for reactivation Hafnia alvei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.3.1.1 10 35 no reactivation between 0-10°C, linear increase at 10-25°C, with a level off between 25-35°C and completely stopped at 35°C Hafnia alvei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.1 7
-
 optimum for reactivation Hafnia alvei