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Literature summary extracted from

  • Miller, O.J.; Hibbert, E.G.; Ingram, C.U.; Lye, G.J.; Dalby, P.A.
    Optimisation and evaluation of a generic microplate-based HPLC screen for transketolase activity (2007), Biotechnol. Lett., 29, 1759-1770.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.2.1.1 additional information the host strain producing the greatest amount of transketolase activity is Escherichia coli strain XL10-gold, which produces 2.3fold more transketolase than the JM107 strain Escherichia coli

Application

EC Number Application Comment Organism
2.2.1.1 analysis establishment of a rapid microplate-based HPLC assay for transketolase, for rapidly determining substrate and product concentration suitable for optimisation of biocatalytic process conditions and screening directed evolution libraries, which can be used to determine transketolase activity with a throughput of up to 1200 samples per day, whereas the well-to-well variation from HPLC measurement is just 1.9% for the lowest activities measured Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.1 into plasmid pQR711 and expressed in Escherichia coli strains BL21-Gold(DE3), JM107, JM109 and XL10-Gold Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.1 additional information Escherichia coli BL21-Gold(DE3) pQR711 has transketolase activity of ca. 6fold lower than JM107 pQR711 and JM109 pQR711 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 Mg2+
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 hydroxypyruvate + D-glyceraldehyde 3-phosphate
-
 Escherichia coli CO2 + ribulose 5-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.1 TktA
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate
-
 Escherichia coli