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Literature summary extracted from

  • Urabe, H.; Aoyagi, N.; Ogawara, H.; Motojima, K.
    Expression and characterization of the Streptomyces coelicolor serine/threonine protein kinase PkaD (2008), Biosci. Biotechnol. Biochem., 72, 778-785.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 expressed in Escherichia coli as a His-tagged fusion protein Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.1 K42N in vitro phosphorylation assay shows that mutant protein is not able to phosphorylate itself Streptomyces coelicolor
2.7.11.1 K42R in vitro phosphorylation assay shows that mutant protein is not able to phosphorylate itself Streptomyces coelicolor
2.7.11.1 additional information disruption of chromosomal pkaD results in a significant loss of actinorhodin production. This result implies the involvement of pkaD in the regulation of secondary metabolism Streptomyces coelicolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.1 membrane a hydrophobicity plot indicates the presence of a putative transmembrane spanning sequence downstream of the catalytic domain, suggesting that PkaD is a transmembrane protein kinase Streptomyces coelicolor 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 63900
-
 deduced from cDNA Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Streptomyces coelicolor O83032
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.1 phosphoprotein PkaD is found to be phosphorylated by itsself at the threonine and tyrosine residues Streptomyces coelicolor

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 using Ni-NTA chromatography Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 PkaD
-
 Streptomyces coelicolor
2.7.11.1 serine-threonine kinase
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 30
-
 assay at Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 7.5
-
 assay at Streptomyces coelicolor