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Literature summary extracted from

  • Semba, H.; Dobashi, Y.; Matsui, T.
    Expression of hydroxynitrile lyase from Manihot esculenta in yeast and its application in (S)-mandelonitrile production using an immobilized enzyme reactor (2008), Biosci. Biotechnol. Biochem., 72, 1457-1463.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.2.47 synthesis (S)-mandelonitrile production on a commercial scale Manihot esculenta

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.47 high expression in a multi-auxotrophic mutant of Saccharomyces cerevisiae Manihot esculenta

General Stability

EC Number General Stability Organism
4.1.2.47 the immobilized enzyme is successfully used for more than 20 repeated batches with no loss of conversion rate or enantioselectivity Manihot esculenta

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.47 Manihot esculenta P52705
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.47 leaf
-
 Manihot esculenta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.47 cyanide + benzaldehyde concentrations of benzaldehyde higher than 1 M result in decreased enantioselectivity due to nonenzymatic formation of racemic mandelonitrile due to an excess of benzaldehyde and cyanide Manihot esculenta (S)-mandelonitrile
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.47 (S)-cyanohydrin producing hydroxynitrile lyase
-
 Manihot esculenta
4.1.2.47 Hydroxynitrile lyase
-
 Manihot esculenta
4.1.2.47 MeHNL
-
 Manihot esculenta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.47 5.5
-
 immobilized enzyme Manihot esculenta