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Literature summary extracted from

  • Sharma, S.; Agarwal, L.; Saxena, R.K.
    Purification, immobilization and characterization of tannase from Penicillium variable (2008), Bioresour. Technol., 99, 2544-2551.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 sodium cholate
-
 Talaromyces variabilis
3.1.1.20 sodium taurocholate
-
 Talaromyces variabilis

General Stability

EC Number General Stability Organism
3.1.1.20 the tannase can successfully be immobilized on Amberlite IR where it retains about 85% of the initial catalytic activity even after the ninth cycle of its use Talaromyces variabilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 1,10-phenanthroline 40% residual activity at 1 mM Talaromyces variabilis
3.1.1.20 1-[(naphthalen-1-yl)oxy]-3-[(propan-2-yl)amino]propan-2-ol 49.6% residual activity at 1 mM Talaromyces variabilis
3.1.1.20 8-hydroxyquinoline 47.2% residual activity at 1 mM Talaromyces variabilis
3.1.1.20 Bromoacetic acid 53.3% residual activity at 1 mM Talaromyces variabilis
3.1.1.20 cyanamide 61.7% residual activity at 1 mM Talaromyces variabilis
3.1.1.20 dithiothreitol 54.1% residual activity at 1 mM Talaromyces variabilis
3.1.1.20 iodoacetic acid 48% residual activity at 1 mM Talaromyces variabilis
3.1.1.20 mercuribenzoic acid 48.8% residual activity at 1 mM Talaromyces variabilis
3.1.1.20 N-ethylmaleimide 7% residual activity at 1 mM Talaromyces variabilis
3.1.1.20 phenyl boronic acid 67.1% residual activity at 1 mM Talaromyces variabilis
3.1.1.20 phenylmethylsulfonyl fluoride 28% residual activity at 1 mM Talaromyces variabilis
3.1.1.20 sodium cholate 52.6% residual activity at 1 mM Talaromyces variabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 12
-
 propyl gallate in 0.5 M citrate phosphate buffer, pH 5.0, at 50°C Talaromyces variabilis
3.1.1.20 14
-
 methyl gallate in 0.5 M citrate phosphate buffer, pH 5.0, at 50°C Talaromyces variabilis
3.1.1.20 32
-
 Tannic acid in 0.5 M citrate phosphate buffer, pH 5.0, at 50°C Talaromyces variabilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 155000
-
 2 * 155000, gel filtration Talaromyces variabilis
3.1.1.20 158000
-
 2 * 158000, SDS-PAGE Talaromyces variabilis
3.1.1.20 310000
-
 gel filtration Talaromyces variabilis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.20 2-mercaptomethanol 39.6% residual activity at 1 mM Talaromyces variabilis
3.1.1.20 Acetone 49% residual activity after 60 min at 20% (v/v) Talaromyces variabilis
3.1.1.20 carbon tetrachloride 57% residual activity after 60 min at 60% (v/v) Talaromyces variabilis
3.1.1.20 Ethanol 35.7% residual activity after 60 min at 60% (v/v) Talaromyces variabilis
3.1.1.20 formaldehyde 12% residual activity after 5 min at 20% (v/v) Talaromyces variabilis
3.1.1.20 heptane 62% residual activity after 60 min at 60% (v/v) Talaromyces variabilis
3.1.1.20 additional information not affected by Triton X-100 Talaromyces variabilis
3.1.1.20 petroleum ether 33% residual activity after 60 min at 60% (v/v) Talaromyces variabilis
3.1.1.20 SDS complete inactivation at 1% (w/v) Talaromyces variabilis
3.1.1.20 tetrahydrofuran no activity after 5 min at 20% (v/v) Talaromyces variabilis
3.1.1.20 toluene 44% residual activity after 60 min at 60% (v/v) Talaromyces variabilis
3.1.1.20 Tween 60 complete inactivation at 1% (v/v) Talaromyces variabilis
3.1.1.20 Tween 80 complete inactivation at 1% (v/v) Talaromyces variabilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Talaromyces variabilis
-
-
-
3.1.1.20 Talaromyces variabilis IARI 2031
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 ultrafiltration and Sephadex G-200 gel filtration Talaromyces variabilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 15.27
-
 crude filtrate, in 0.5 M citrate phosphate buffer, pH 5.0, at 50°C Talaromyces variabilis
3.1.1.20 2055
-
 after 135fold purification, in 0.5 M citrate phosphate buffer, pH 5.0, at 50°C Talaromyces variabilis

Storage Stability

EC Number Storage Stability Organism
3.1.1.20 4°C and 30°C, tannase in both crude and crude lyophilized forms, one year, retains more than 60% residual activity Talaromyces variabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 methyl gallate + H2O
-
 Talaromyces variabilis gallic acid + methanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Talaromyces variabilis IARI 2031 gallic acid + methanol
-
 ?
3.1.1.20 n-propyl gallate + H2O
-
 Talaromyces variabilis gallic acid + n-propanol
-
 ?
3.1.1.20 n-propyl gallate + H2O
-
 Talaromyces variabilis IARI 2031 gallic acid + n-propanol
-
 ?
3.1.1.20 tannic acid + H2O best substrate Talaromyces variabilis gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O best substrate Talaromyces variabilis IARI 2031 gallate + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 homodimer 2 * 158000, SDS-PAGE Talaromyces variabilis
3.1.1.20 homodimer 2 * 155000, gel filtration Talaromyces variabilis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acyl hydrolase belongs to the class of serine hydrolases Talaromyces variabilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 50
-
-
 Talaromyces variabilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.20 25 80
-
 Talaromyces variabilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 25 90 at 25°C crude and immobilized enzyme show 78% and 81% relative activity while purified tannase has a relative activity of 70% at this temperature, at 80°C more than 50% relative activity is observed in crude and immobilized tannase while 45% in purified form, at 90°C crude and immobilized enzyme retain 23% and 31% relative activity while purified tannase shows a relative activity of only 11% Talaromyces variabilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 5
-
-
 Talaromyces variabilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.20 3 8
-
 Talaromyces variabilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.20 3 10 the enzyme shows 60% residual activity at pH 3.0, almost 100% stability in pH range of 4.0-6.0 for 24 h, at pH 10.0 crude and purified tannase retain 19.7% and 15.3% residual activity for 24 h, immobilized tannase retains 24.6% residual activity at this pH even after 24 h Talaromyces variabilis