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Literature summary extracted from

  • Li, N.; Chu, X.; Liu, X.; Li, D.
    Probing the active site of rat porphobilinogen synthase using newly developed inhibitors (2009), Bioorg. Chem., 37, 33-40.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.24 hexa-His-tag, removed by cleavage with thrombin Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.24 (4E)-6-oxodec-4-enedioic acid
-
 Rattus sp.
4.2.1.24 1-amino-4-hydroxy-2-butanone
-
 Rattus sp.
4.2.1.24 1-amino-4-methoxy-2-butanone
-
 Rattus sp.
4.2.1.24 1-amino-5-hydroxy-2-pentanone
-
 Rattus sp.
4.2.1.24 4-oxo-pentanenitrile
-
 Rattus sp.
4.2.1.24 4-oxo-sebacic acid
-
 Rattus sp.
4.2.1.24 5-amino-4-oxopentanenitrile
-
 Rattus sp.
4.2.1.24 5-bromo-levulinic acid
-
 Rattus sp.
4.2.1.24 5-oxo-hexanoic acid
-
 Rattus sp.
4.2.1.24 6-amino-5-oxohexanoic acid
-
 Rattus sp.
4.2.1.24 EDTA
-
 Rattus sp.
4.2.1.24 levulinic acid
-
 Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.24 0.65
-
 5-aminolevulinic acid expressed from expression vector pET28a+ Rattus sp.
4.2.1.24 2.1
-
 5-aminolevulinic acid expressed from expression vector pET28a+, His-tag removed Rattus sp.
4.2.1.24 3.22
-
 5-aminolevulinic acid expressed from expression vector pLM1 Rattus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.24 Zn2+
-
 Rattus sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.24 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.24 apparent homogeneity (analyzed by SDS-PAGE) Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.24 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.24 0.09
-
 expressed from expression vector pLM1 Rattus sp.
4.2.1.24 0.28
-
 expressed from expression vector pET28a+ Rattus sp.
4.2.1.24 0.29
-
 expressed from expression vector pET28a+, His-tag removed Rattus sp.

Storage Stability

EC Number Storage Stability Organism
4.2.1.24 -80øC, one year Rattus sp.
4.2.1.24 4øC, one week, without significant change of activity Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.24 5-aminolevulinic acid + 5-aminolevulinic acid
-
 Rattus sp. porphobilinogen + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.24 5-aminolaevulinic acid dehydratase
-
 Rattus sp.
4.2.1.24 Porphobilinogen synthase
-
 Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.24 8.5
-
-
 Rattus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.24 0.96
-
 levulinic acid
-
 Rattus sp.
4.2.1.24 1.07
-
 6-amino-5-oxohexanoic acid
-
 Rattus sp.
4.2.1.24 2.93
-
 4-oxo-sebacic acid
-
 Rattus sp.
4.2.1.24 3.05
-
 5-oxo-hexanoic acid
-
 Rattus sp.
4.2.1.24 18.48
-
 5-amino-4-oxopentanenitrile
-
 Rattus sp.
4.2.1.24 26.52
-
 1-amino-4-hydroxy-2-butanone
-
 Rattus sp.
4.2.1.24 73.04
-
 4-oxo-pentanenitrile
-
 Rattus sp.

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.24 30
-
-
 Rattus sp. 1-amino-4-methoxy-2-butanone
4.2.1.24 32.5
-
-
 Rattus sp. 1-amino-5-hydroxy-2-pentanone