BRENDA - Enzyme Database show

Probing the active site of rat porphobilinogen synthase using newly developed inhibitors

Li, N.; Chu, X.; Liu, X.; Li, D.; Bioorg. Chem. 37, 33-40 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.24
hexa-His-tag, removed by cleavage with thrombin
Rattus sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
(4E)-6-oxodec-4-enedioic acid
-
Rattus sp.
4.2.1.24
1-amino-4-hydroxy-2-butanone
-
Rattus sp.
4.2.1.24
1-amino-4-methoxy-2-butanone
-
Rattus sp.
4.2.1.24
1-amino-5-hydroxy-2-pentanone
-
Rattus sp.
4.2.1.24
4-oxo-pentanenitrile
-
Rattus sp.
4.2.1.24
4-oxo-sebacic acid
-
Rattus sp.
4.2.1.24
5-amino-4-oxopentanenitrile
-
Rattus sp.
4.2.1.24
5-bromo-levulinic acid
-
Rattus sp.
4.2.1.24
5-oxo-hexanoic acid
-
Rattus sp.
4.2.1.24
6-amino-5-oxohexanoic acid
-
Rattus sp.
4.2.1.24
EDTA
-
Rattus sp.
4.2.1.24
levulinic acid
-
Rattus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.65
-
5-aminolevulinic acid
expressed from expression vector pET28a+
Rattus sp.
4.2.1.24
2.1
-
5-aminolevulinic acid
expressed from expression vector pET28a+, His-tag removed
Rattus sp.
4.2.1.24
3.22
-
5-aminolevulinic acid
expressed from expression vector pLM1
Rattus sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Zn2+
-
Rattus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Rattus sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
apparent homogeneity (analyzed by SDS-PAGE)
Rattus sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.24
liver
-
Rattus sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.24
0.09
-
expressed from expression vector pLM1
Rattus sp.
4.2.1.24
0.28
-
expressed from expression vector pET28a+
Rattus sp.
4.2.1.24
0.29
-
expressed from expression vector pET28a+, His-tag removed
Rattus sp.
Storage Stability
EC Number
Storage Stability
Organism
4.2.1.24
-80°C, one year
Rattus sp.
4.2.1.24
4°C, one week, without significant change of activity
Rattus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinic acid + 5-aminolevulinic acid
-
691205
Rattus sp.
porphobilinogen + 2 H2O
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
8.5
-
-
Rattus sp.
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.24
0.96
-
levulinic acid
-
Rattus sp.
4.2.1.24
1.07
-
6-amino-5-oxohexanoic acid
-
Rattus sp.
4.2.1.24
2.93
-
4-oxo-sebacic acid
-
Rattus sp.
4.2.1.24
3.05
-
5-oxo-hexanoic acid
-
Rattus sp.
4.2.1.24
18.48
-
5-amino-4-oxopentanenitrile
-
Rattus sp.
4.2.1.24
26.52
-
1-amino-4-hydroxy-2-butanone
-
Rattus sp.
4.2.1.24
73.04
-
4-oxo-pentanenitrile
-
Rattus sp.
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.2.1.24
30
-
-
Rattus sp.
1-amino-4-methoxy-2-butanone
4.2.1.24
32.5
-
-
Rattus sp.
1-amino-5-hydroxy-2-pentanone
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
hexa-His-tag, removed by cleavage with thrombin
Rattus sp.
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.2.1.24
30
-
-
Rattus sp.
1-amino-4-methoxy-2-butanone
4.2.1.24
32.5
-
-
Rattus sp.
1-amino-5-hydroxy-2-pentanone
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
(4E)-6-oxodec-4-enedioic acid
-
Rattus sp.
4.2.1.24
1-amino-4-hydroxy-2-butanone
-
Rattus sp.
4.2.1.24
1-amino-4-methoxy-2-butanone
-
Rattus sp.
4.2.1.24
1-amino-5-hydroxy-2-pentanone
-
Rattus sp.
4.2.1.24
4-oxo-pentanenitrile
-
Rattus sp.
4.2.1.24
4-oxo-sebacic acid
-
Rattus sp.
4.2.1.24
5-amino-4-oxopentanenitrile
-
Rattus sp.
4.2.1.24
5-bromo-levulinic acid
-
Rattus sp.
4.2.1.24
5-oxo-hexanoic acid
-
Rattus sp.
4.2.1.24
6-amino-5-oxohexanoic acid
-
Rattus sp.
4.2.1.24
EDTA
-
Rattus sp.
4.2.1.24
levulinic acid
-
Rattus sp.
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.24
0.96
-
levulinic acid
-
Rattus sp.
4.2.1.24
1.07
-
6-amino-5-oxohexanoic acid
-
Rattus sp.
4.2.1.24
2.93
-
4-oxo-sebacic acid
-
Rattus sp.
4.2.1.24
3.05
-
5-oxo-hexanoic acid
-
Rattus sp.
4.2.1.24
18.48
-
5-amino-4-oxopentanenitrile
-
Rattus sp.
4.2.1.24
26.52
-
1-amino-4-hydroxy-2-butanone
-
Rattus sp.
4.2.1.24
73.04
-
4-oxo-pentanenitrile
-
Rattus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.65
-
5-aminolevulinic acid
expressed from expression vector pET28a+
Rattus sp.
4.2.1.24
2.1
-
5-aminolevulinic acid
expressed from expression vector pET28a+, His-tag removed
Rattus sp.
4.2.1.24
3.22
-
5-aminolevulinic acid
expressed from expression vector pLM1
Rattus sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Zn2+
-
Rattus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
apparent homogeneity (analyzed by SDS-PAGE)
Rattus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.24
liver
-
Rattus sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.24
0.09
-
expressed from expression vector pLM1
Rattus sp.
4.2.1.24
0.28
-
expressed from expression vector pET28a+
Rattus sp.
4.2.1.24
0.29
-
expressed from expression vector pET28a+, His-tag removed
Rattus sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.2.1.24
-80°C, one year
Rattus sp.
4.2.1.24
4°C, one week, without significant change of activity
Rattus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinic acid + 5-aminolevulinic acid
-
691205
Rattus sp.
porphobilinogen + 2 H2O
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
8.5
-
-
Rattus sp.