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Literature summary extracted from

  • Abe, K.; Mihara, H.; Nishijima, Y.; Kurokawa, S.; Esaki, N.
    Functional analysis of two homologous mouse selenophosphate synthetases (2008), Biomed. Res. Trace Elem., 19, 76-79.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.3 expression of His-tagged SPS1 and of His-tagged SPS2 mutant Sec69Cys in Escherichia coli strain Rosetta (DE3) Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.9.3 Sec63C site-directed mutagenesis, SPS2 mutant Sec63Cys catalyzes the selenide-dependent synthesis of selenophosphate Mus musculus
2.7.9.3 T29C site-directed mutagenesis, the Thr29Cys mutant of SPS1 exhibits SPS activity Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.3 Mg2+
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.3 ATP + selenide Mus musculus SPS2 AMP + selenophosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.3 Mus musculus P97364
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.3 recombinant His-tagged SPS1 and His-tagged SPS2 mutant Sec69Cys from Escherichia coli strain Rosetta (DE3) to homogeneity by affinity chromatography on a His-binding resin Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.3 ATP + selenide SPS2 Mus musculus AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 ATP + selenide SPS2, the catalytically important selenocysteine residue is located at position 63 Mus musculus AMP + selenophosphate + phosphate
-
 ?
2.7.9.3 additional information SPS1 shows no activity Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.9.3 selenophosphate synthetase 2
-
 Mus musculus
2.7.9.3 Sps2
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.9.3 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.9.3 7.4
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.9.3 ATP
-
 Mus musculus