Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fischer, N.; Paleskava, A.; Gromadski, K.B.; Konevega, A.L.; Wahl, M.C.; Stark, H.; Rodnina, M.V.
    Towards understanding selenocysteine incorporation into bacterial proteins (2007), Biol. Chem., 388, 1061-1067.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.9.1.1 selA, expression in Escherichia coli strain DH5alpha Moorella thermoacetica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9.1.1 additional information
-
 additional information kinetics study of selenocysteine incorporation Moorella thermoacetica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.9.1.1 L-seryl-tRNASec + selenophosphate Moorella thermoacetica the enzyme is involved in selenocysteine incorporation into proteins in bacteria, pathway overview L-selenocysteinyl-tRNASec + phosphate
-
 ?
2.9.1.1 additional information Moorella thermoacetica SelA interacts with the transcription factor SelB, mutational interaction study, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.9.1.1 Moorella thermoacetica
-
 gene selA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.9.1.1 recombinant SelA from Escherichia coli strain DH5alpha by anion exchange chromatography, ammonium sulfate fractionation, and gel filtration Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.9.1.1 L-seryl-tRNASec + selenophosphate
-
 Moorella thermoacetica L-selenocysteinyl-tRNASec + phosphate
-
 ?
2.9.1.1 L-seryl-tRNASec + selenophosphate the enzyme is involved in selenocysteine incorporation into proteins in bacteria, pathway overview Moorella thermoacetica L-selenocysteinyl-tRNASec + phosphate
-
 ?
2.9.1.1 additional information SelA interacts with the transcription factor SelB, mutational interaction study, overview Moorella thermoacetica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.9.1.1 decamer the homodecamer has a ring-like structure with five bilobed wings, similar to the structure of the Escherichia coli complex Moorella thermoacetica
2.9.1.1 More formation of the ternary enzyme complex and binding to the ribosome, structure analysis by electron microscopy, 5fold rotational symmetry, overview Moorella thermoacetica

Synonyms

EC Number Synonyms Comment Organism
2.9.1.1 SelA
-
 Moorella thermoacetica
2.9.1.1 Selenocysteine synthase
-
 Moorella thermoacetica