Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Xiong, X.; Cui, P.; Hossain, S.; Xu, R.; Warner, B.; Guo, X.; An, X.; Debnath, A.K.; Cowburn, D.; Kotula, L.
    Allosteric inhibition of the nonMyristoylated c-Abl tyrosine kinase by phosphopeptides derived from Abi1/Hssh3bp1 (2008), Biochim. Biophys. Acta, 1783, 737-747.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.10.2 His-tagged, partially capped, active, nonmyristoylated c-Abl, E46 through C-terminus, isoform 1b numbering, is produced, GST fusions of c-Abl SH3 and SH2 domains are used, the dual domain SH3-SH2 is expressed from plasmid pTXB1 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.10.2 R171K SH2 domain variant containing a mutation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.10.2 additional information pY213 phosphopeptide of Abi1 inhibits c-Abl kinase activity by a noncompetitive mechanism Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.10.2 ATP + [protein]-L-tyrosine Homo sapiens
-
 ADP + [protein]-L-tyrosine phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.10.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.10.2 affinity purified on nickel-nitriloacetic acid agarose and by ion-exchange chromatography using a Mono S column, through chitin affinitive binding and by SP Sepharose cation exchange Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.10.2 ATP + GGEAIYAAPFKK kinase assay using biotinylated model substrate peptide Homo sapiens ADP + GGEAIYAAPFKK phosphate
-
 ?
2.7.10.2 ATP + [Abi1]-L-tyrosine c-Abl phosphorylates Y213 of Abi1 Homo sapiens ADP + [Abi1]-L-tyrosine phosphate
-
 ?
2.7.10.2 ATP + [protein]-L-tyrosine
-
 Homo sapiens ADP + [protein]-L-tyrosine phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.10.2 c-Abl tyrosine kinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.10.2 30
-
 kinase assay Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.10.2 7.5
-
 kinase assay Homo sapiens