EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.99.11 | expression in Escherichia coli BL21DE3 cells, the vectors pET-28b, pACYC-Duet, pET-Duet and pRSF-Duet are used | Thauera aromatica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.99.11 | 7000 | - |
gamma subunit, determined by SDS-PAGE | Thauera aromatica |
4.1.99.11 | 14000 | - |
beta subunit, determined by SDS-PAGE | Thauera aromatica |
4.1.99.11 | 100000 | - |
alpha subunit, determined by SDS-PAGE | Thauera aromatica |
4.1.99.11 | 230000 | - |
determined by gel filtration, consistent with an alpha2beta2gamma2 subunit structure | Thauera aromatica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.11 | toluene + fumarate | Thauera aromatica | - |
benzylsuccinate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.11 | Thauera aromatica | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.99.11 | using a Ni-NTA-agarose column | Thauera aromatica |
EC Number | Renatured (Comment) | Organism |
---|---|---|
4.1.99.11 | the alpha subunit is purified and refolded from inclusion bodies | Thauera aromatica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.11 | toluene + fumarate | - |
Thauera aromatica | benzylsuccinate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.99.11 | heterotrimer | alpha, beta and gamma subunit | Thauera aromatica |
4.1.99.11 | hexamer | the structural model for benzylsuccinate synthase proposes an alpha2beta2gamma2-complex | Thauera aromatica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.99.11 | benzylsuccinate synthase | - |
Thauera aromatica |
4.1.99.11 | BSS | - |
Thauera aromatica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.99.11 | iron-sulfur centre | - |
Thauera aromatica |