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Literature summary extracted from
- Backbone flexibility, conformational change, and catalysis in a phosphohexomutase from Pseudomonas aeruginosa (2008), Biochemistry, 47, 9154-9162.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.2.8 | expressed in Escherichia coli | Pseudomonas aeruginosa |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.4.2.8 | mutant enzyme P368G, hanging drop vapour diffusion method | Pseudomonas aeruginosa |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.2.8 | E325A | mutant shows 0.08% of wild type activity | Pseudomonas aeruginosa |
| 5.4.2.8 | P368A | mutant shows 10% of wild type activity | Pseudomonas aeruginosa |
| 5.4.2.8 | P368G | mutant shows 8.7% of wild type activity | Pseudomonas aeruginosa |
| 5.4.2.8 | R262A | mutant shows 6.1% of wild type activity | Pseudomonas aeruginosa |
| 5.4.2.8 | R262A/P368G | mutant shows 2.5% of wild type activity | Pseudomonas aeruginosa |
| 5.4.2.8 | S369A | mutant shows 20.5% of wild type activity | Pseudomonas aeruginosa |
| 5.4.2.8 | Y17A | mutant shows 0.35% of wild type activity | Pseudomonas aeruginosa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.8 | 0.0272 | - |
alpha-D-glucose 1-phosphate | wild type enzyme, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa |  |
| 5.4.2.8 | 0.0354 | - |
alpha-D-glucose 1-phosphate | mutant enzyme P368A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 0.0366 | - |
alpha-D-glucose 1-phosphate | mutant enzyme S369A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 0.0489 | - |
alpha-D-glucose 1-phosphate | mutant enzyme P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 0.0491 | - |
alpha-D-glucose 1-phosphate | mutant enzyme R262A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 0.0493 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y17A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 0.0518 | - |
alpha-D-glucose 1-phosphate | mutant enzyme E325A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 0.0667 | - |
alpha-D-glucose 1-phosphate | mutant enzyme R262A/P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.2.8 | Pseudomonas aeruginosa | P26276 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.2.8 | - |
Pseudomonas aeruginosa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.8 | alpha-D-glucose 1-phosphate | - |
Pseudomonas aeruginosa | alpha-D-glucose 6-phosphate | - |
? | |
| 5.4.2.8 | alpha-D-mannose 1-phosphate | - |
Pseudomonas aeruginosa | alpha-D-mannose 6-phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.2.8 | phosphomannomutase/phosphoglucomutase | - |
Pseudomonas aeruginosa |
| 5.4.2.8 | PMM/PGM | bifunctional enzyme | Pseudomonas aeruginosa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.8 | 0.012 | - |
alpha-D-glucose 1-phosphate | mutant enzyme E325A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 0.05 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y17A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 0.48 | - |
alpha-D-glucose 1-phosphate | mutant enzyme R262A/P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 0.87 | - |
alpha-D-glucose 1-phosphate | mutant enzyme R262A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 1.02 | - |
alpha-D-glucose 1-phosphate | mutant enzyme P368A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 1.23 | - |
alpha-D-glucose 1-phosphate | mutant enzyme P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 2.17 | - |
alpha-D-glucose 1-phosphate | mutant enzyme S369A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
| 5.4.2.8 | 7.83 | - |
alpha-D-glucose 1-phosphate | wild type enzyme, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
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