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Literature summary extracted from
- Key residues of loop 3 in the interaction with the interface residue at position 14 in triosephosphate isomerase from Trypanosoma brucei (2008), Biochemistry, 47, 3499-3506.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.1.1 | C14S | wild-type-like enzyme that is resistant to the action of sulfhydryl reagents methylmethane thiosulfonate and 5,5-dithiobis(2-nitrobenzoate) | Trypanosoma brucei |
| 5.3.1.1 | C14S/A69C | similar in kinetic parameters to wild-type TbTIM and the single mutant C14S. Mutant binds 50 times more 1-anilino-8-naphthalene sulfonate than wild-type and is susceptible to digestion with subtilisin | Trypanosoma brucei |
| 5.3.1.1 | C14S/A73C | greatly reduced kcat value. Mutant binds 50 times more 1-anilino-8-naphthalene sulfonate than wild-type and is susceptible to digestion with subtilisin | Trypanosoma brucei |
| 5.3.1.1 | C14S/S71C | similar in kinetic parameters to wild-type TbTIM and the single mutant C14S. Mutant binds 50 times more 1-anilino-8-naphthalene sulfonate than wild-type and is susceptible to digestion with subtilisin | Trypanosoma brucei |
| 5.3.1.1 | C14S/S79C | similar in kinetic parameters to wild-type TbTIM and the single mutant C14S. Mutant binds 50 times more 1-anilino-8-naphthalene sulfonate than wild-type and is susceptible to digestion with subtilisin | Trypanosoma brucei |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.1 | 0.14 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/A73C, 25°C, pH 7.4 | Trypanosoma brucei |  |
| 5.3.1.1 | 0.35 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/S71C, 25°C, pH 7.4 | Trypanosoma brucei | |
| 5.3.1.1 | 0.38 | - |
D-glyceraldehyde 3-phosphate | wild-type, 25°C, pH 7.4 | Trypanosoma brucei | |
| 5.3.1.1 | 0.48 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/S79C, 25°C, pH 7.4 | Trypanosoma brucei | |
| 5.3.1.1 | 0.5 | - |
D-glyceraldehyde 3-phosphate | mutant C14S, 25°C, pH 7.4 | Trypanosoma brucei | |
| 5.3.1.1 | 0.75 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/A69C, 25°C, pH 7.4 | Trypanosoma brucei | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.1 | Trypanosoma brucei | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.1 | D-glyceraldehyde 3-phosphate | - |
Trypanosoma brucei | dihydroxyacetone phosphate | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.1 | 483 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/A73C, 25°C, pH 7.4 | Trypanosoma brucei | |
| 5.3.1.1 | 2600 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/A69C, 25°C, pH 7.4 | Trypanosoma brucei | |
| 5.3.1.1 | 2967 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/S79C, 25°C, pH 7.4 | Trypanosoma brucei | |
| 5.3.1.1 | 3200 | - |
D-glyceraldehyde 3-phosphate | mutant C14S/S71C, 25°C, pH 7.4 | Trypanosoma brucei | |
| 5.3.1.1 | 4183 | - |
D-glyceraldehyde 3-phosphate | wild-type, 25°C, pH 7.4 | Trypanosoma brucei | |
| 5.3.1.1 | 5133 | - |
D-glyceraldehyde 3-phosphate | mutant C14S, 25°C, pH 7.4 | Trypanosoma brucei | |
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