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Kinetics and thermodynamics of the interchange of the morpheein forms of human porphobilinogen synthase

Selwood, T.; Tang, L.; Lawrence, S.H.; Anokhina, Y.; Jaffe, E.K.; Biochemistry 47, 3245-3257 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.24
F12L
naturally occurring variant
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.02
-
5-aminolevulinic acid
wild type, pH 9, octamer
Homo sapiens
4.2.1.24
0.2
-
5-aminolevulinic acid
wild type, pH 7, almost octamer
Homo sapiens
4.2.1.24
4.5
-
5-aminolevulinic acid
wild type, pH 9, predominantly hexamer and/or dimer
Homo sapiens
4.2.1.24
4.6
-
5-aminolevulinic acid
F12L, pH 9
Homo sapiens
4.2.1.24
18
-
5-aminolevulinic acid
F12L, pH 7
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Homo sapiens
P13716
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.24
0.67
-
wild-type octamer
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinic acid + 5-aminolevulinic acid
-
690939
Homo sapiens
porphobilinogen + 2 H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.24
dimer
pro-hexamer dimer and pro-octamer dimer, analyzed by gel filtration
Homo sapiens
4.2.1.24
hexamer
wild type, pH 8.8, catalytic turnover favors octamer, analyzed by gel filtration and anion exchange chromatography
Homo sapiens
4.2.1.24
octamer
wild type, pH 6.9 and pH 8.8, analyzed by gel filtration and anion exchange chromatography
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.24
F12L
naturally occurring variant
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.02
-
5-aminolevulinic acid
wild type, pH 9, octamer
Homo sapiens
4.2.1.24
0.2
-
5-aminolevulinic acid
wild type, pH 7, almost octamer
Homo sapiens
4.2.1.24
4.5
-
5-aminolevulinic acid
wild type, pH 9, predominantly hexamer and/or dimer
Homo sapiens
4.2.1.24
4.6
-
5-aminolevulinic acid
F12L, pH 9
Homo sapiens
4.2.1.24
18
-
5-aminolevulinic acid
F12L, pH 7
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.24
0.67
-
wild-type octamer
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinic acid + 5-aminolevulinic acid
-
690939
Homo sapiens
porphobilinogen + 2 H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.24
dimer
pro-hexamer dimer and pro-octamer dimer, analyzed by gel filtration
Homo sapiens
4.2.1.24
hexamer
wild type, pH 8.8, catalytic turnover favors octamer, analyzed by gel filtration and anion exchange chromatography
Homo sapiens
4.2.1.24
octamer
wild type, pH 6.9 and pH 8.8, analyzed by gel filtration and anion exchange chromatography
Homo sapiens