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Literature summary extracted from

  • Pfoestl, A.; Zayni, S.; Hofinger, A.; Kosma, P.; Schaeffer, C.; Messner, P.
    Biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha -D-glucose (2008), Biochem. J., 410, 187-194.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.46 expressed in Escherichia coli BL21(DE3) and DH5alpha Thermoanaerobacterium thermosaccharolyticum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.46 dTDP-glucose Thermoanaerobacterium thermosaccharolyticum involved in initial steps of glycan biosynthesis of bacterial cell surface layer (S-layer) dTDP-4-dehydro-6-deoxy-D-glucose + H2O
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 ?
4.2.1.46 dTDP-glucose Thermoanaerobacterium thermosaccharolyticum E207-71 involved in initial steps of glycan biosynthesis of bacterial cell surface layer (S-layer) dTDP-4-dehydro-6-deoxy-D-glucose + H2O
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 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.46 Thermoanaerobacterium thermosaccharolyticum Q6TFC2 rmlB gene; E207-71
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4.2.1.46 Thermoanaerobacterium thermosaccharolyticum E207-71 Q6TFC2 rmlB gene; E207-71
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Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.46 gel filtration, SDS-PAGE Thermoanaerobacterium thermosaccharolyticum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.46 additional information
-
 involved in initial steps of biosynthesis of the S-layer glycan component dTDP-3-acetamido-3,6-dideoxy-alpha-D-glucose (Quip3NAc), chromosomal organization shown, gene homologues in Gram-negative as well as in antibiotic-producing Gram-positive bacteria, also key enzyme in the biosynthetic pathway of dTDP-beta-L-rhamnose, biosynthesis pathway may also be valid for LPS (lipopolysaccharide) O-antigen structures and antibiotic precursors Thermoanaerobacterium thermosaccharolyticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.46 dTDP-glucose involved in initial steps of glycan biosynthesis of bacterial cell surface layer (S-layer) Thermoanaerobacterium thermosaccharolyticum dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
4.2.1.46 dTDP-glucose involved in initial steps of glycan biosynthesis of bacterial cell surface layer (S-layer) Thermoanaerobacterium thermosaccharolyticum E207-71 dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.46 RmlB
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 Thermoanaerobacterium thermosaccharolyticum