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Literature summary extracted from

  • Suau, T.; Alvaro, G.; Benaiges, M.D.; Lopez-Santin, J.
    Kinetic modelling of aldolase-catalyzed addition between dihydroxyacetone phosphate and (S)-alaninal (2008), Biochem. Eng. J., 41, 95-103.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.17 expression in Escherichia coli XL1 blue MRF cells Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.17 (S)-Cbz-alaninal non-competitive inhibition Escherichia coli
4.1.2.17 methylglyoxal competitive inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.17 54.7
-
 dihydroxyacetone phosphate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.17 24000
-
 monomer Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.17 dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate Escherichia coli
-
 ?
-
 r
4.1.2.17 dihydroxyacetone phosphate + L-lactaldehyde Escherichia coli
-
 L-fuculose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.17 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.17 by metal-chelate affinity chromatography and (NH4)2SO4 precipitation after ZnSO4 incubation Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.17 10
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.17 (S)-Cbz-alaninal + dihydroxyacetone phosphate
-
 Escherichia coli (3R,4R,5S)-5-(benzyloxycarbonylamino)-5,6-dideoxy-1-phosphonohex-2-ulose
-
 ?
4.1.2.17 dihydroxyacetone phosphate
-
 Escherichia coli methylglyoxal + phosphate
-
 ?
4.1.2.17 dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate
-
 Escherichia coli ?
-
 r
4.1.2.17 dihydroxyacetone phosphate + L-lactaldehyde
-
 Escherichia coli L-fuculose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.2.17 tetramer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.1.2.17 FucA
-
 Escherichia coli
4.1.2.17 fuculose-1-phosphate aldolase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.17 25
-
 FucA activity assay Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.17 0.36
-
 dihydroxyacetone phosphate
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.17 7.5
-
 FucA activity assay Escherichia coli