Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ambo, T.; Noike, M.; Kurokawa, H.; Koyama, T.
    Cloning and functional analysis of novel short-chain cis-prenyltransferases (2008), Biochem. Biophys. Res. Commun., 375, 536-540.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.68
-
 Mycobacterium tuberculosis
2.5.1.68 expression in Escherichia coli Corynebacterium glutamicum
2.5.1.68 expression in Escherichia coli Thermobifida fusca
2.5.1.68 expression in Escherichia coli Corynebacterium efficiens

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.68 I59A change in product chain-length, produces prenyl products with chain length up to C50 Thermobifida fusca
2.5.1.68 I59F inactive mutant enzyme Thermobifida fusca
2.5.1.68 I59M change in product chain-length, produces prenyl products with chain length up to C50 Thermobifida fusca
2.5.1.68 I59V change in product chain-length, produces prenyl products with chain length up to C50 Thermobifida fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.68 0.0012
-
 isopentenyl diphosphate pH 7.5, 37°C Thermobifida fusca
2.5.1.68 0.0023
-
 geranyl diphosphate pH 7.5, 37°C Thermobifida fusca
2.5.1.68 0.0027
-
 isopentenyl diphosphate pH 7.5, 37°C Corynebacterium efficiens
2.5.1.68 0.0041
-
 geranyl diphosphate pH 7.5, 37°C Corynebacterium efficiens
2.5.1.68 0.0045
-
 isopentenyl diphosphate pH 7.5, 37°C Mycobacterium tuberculosis
2.5.1.68 0.0067
-
 isopentenyl diphosphate pH 7.5, 37°C Corynebacterium glutamicum
2.5.1.68 0.0068
-
 geranyl diphosphate pH 7.5, 37°C Corynebacterium glutamicum
2.5.1.68 0.0078
-
 geranyl diphosphate pH 7.5, 37°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.68 31000
-
 x * 31000, SDS-PAGE, calculated from sequence Corynebacterium glutamicum
2.5.1.68 31000
-
 x * 31000, SDS-PAGE, calculated from sequence Thermobifida fusca
2.5.1.68 31000
-
 x * 31000, SDS-PAGE, calculated from sequence Corynebacterium efficiens

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.68 Corynebacterium efficiens
-
-
-
2.5.1.68 Corynebacterium glutamicum
-
-
-
2.5.1.68 Mycobacterium tuberculosis
-
-
-
2.5.1.68 Thermobifida fusca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.68
-
 Corynebacterium glutamicum
2.5.1.68
-
 Thermobifida fusca
2.5.1.68
-
 Corynebacterium efficiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.68 geranyl diphosphate + isopentenyl diphosphate only geranyl diphosphate is the acceptable substrate among the three allylic substrates, dimethylallyl diphosphate, geranyl diphosphate, and farnesyl diphosphate Corynebacterium glutamicum diphosphate + (2Z,6E)-farnesyl diphosphate
-
 ?
2.5.1.68 geranyl diphosphate + isopentenyl diphosphate only geranyl diphosphate is the acceptable substrate among the three allylic substrates, dimethylallyl diphosphate, geranyl diphosphate, and farnesyl diphosphate Mycobacterium tuberculosis diphosphate + (2Z,6E)-farnesyl diphosphate
-
 ?
2.5.1.68 geranyl diphosphate + isopentenyl diphosphate only geranyl diphosphate is the acceptable substrate among the three allylic substrates, dimethylallyl diphosphate, geranyl diphosphate, and farnesyl diphosphate Thermobifida fusca diphosphate + (2Z,6E)-farnesyl diphosphate
-
 ?
2.5.1.68 geranyl diphosphate + isopentenyl diphosphate only geranyl diphosphate is the acceptable substrate among the three allylic substrates, dimethylallyl diphosphate, geranyl diphosphate, and farnesyl diphosphate Corynebacterium efficiens diphosphate + (2Z,6E)-farnesyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.68 ? x * 31000, SDS-PAGE, calculated from sequence Corynebacterium glutamicum
2.5.1.68 ? x * 31000, SDS-PAGE, calculated from sequence Thermobifida fusca
2.5.1.68 ? x * 31000, SDS-PAGE, calculated from sequence Corynebacterium efficiens

Synonyms

EC Number Synonyms Comment Organism
2.5.1.68 cis-farnesyl diphosphate synthase
-
 Corynebacterium glutamicum
2.5.1.68 cis-farnesyl diphosphate synthase
-
 Mycobacterium tuberculosis
2.5.1.68 cis-farnesyl diphosphate synthase
-
 Thermobifida fusca
2.5.1.68 cis-farnesyl diphosphate synthase
-
 Corynebacterium efficiens
2.5.1.68 cis-FPS
-
 Corynebacterium glutamicum
2.5.1.68 cis-FPS
-
 Mycobacterium tuberculosis
2.5.1.68 cis-FPS
-
 Thermobifida fusca
2.5.1.68 cis-FPS
-
 Corynebacterium efficiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.68 37
-
 assay at Corynebacterium glutamicum
2.5.1.68 37
-
 assay at Mycobacterium tuberculosis
2.5.1.68 37
-
 assay at Thermobifida fusca
2.5.1.68 37
-
 assay at Corynebacterium efficiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.68 141
-
 geranyl diphosphate pH 7.5, 37°C Corynebacterium efficiens
2.5.1.68 173
-
 isopentenyl diphosphate pH 7.5, 37°C Corynebacterium efficiens
2.5.1.68 229
-
 geranyl diphosphate pH 7.5, 37°C Thermobifida fusca
2.5.1.68 282
-
 isopentenyl diphosphate pH 7.5, 37°C Thermobifida fusca
2.5.1.68 329
-
 isopentenyl diphosphate pH 7.5, 37°C Corynebacterium glutamicum
2.5.1.68 329
-
 geranyl diphosphate pH 7.5, 37°C Corynebacterium glutamicum
2.5.1.68 788
-
 isopentenyl diphosphate pH 7.5, 37°C Mycobacterium tuberculosis
2.5.1.68 860
-
 geranyl diphosphate pH 7.5, 37°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.68 7.5
-
 assay at Corynebacterium glutamicum
2.5.1.68 7.5
-
 assay at Mycobacterium tuberculosis
2.5.1.68 7.5
-
 assay at Thermobifida fusca
2.5.1.68 7.5
-
 assay at Corynebacterium efficiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.68 34390
-
 geranyl diphosphate pH 7.5, 37°C Corynebacterium efficiens
2.5.1.68 48380
-
 geranyl diphosphate pH 7.5, 37°C Corynebacterium glutamicum
2.5.1.68 49100
-
 isopentenyl diphosphate pH 7.5, 37°C Corynebacterium glutamicum
2.5.1.68 64070
-
 isopentenyl diphosphate pH 7.5, 37°C Corynebacterium efficiens
2.5.1.68 99570
-
 geranyl diphosphate pH 7.5, 37°C Thermobifida fusca
2.5.1.68 110300
-
 geranyl diphosphate pH 7.5, 37°C Mycobacterium tuberculosis
2.5.1.68 175100
-
 isopentenyl diphosphate pH 7.5, 37°C Mycobacterium tuberculosis
2.5.1.68 235000
-
 isopentenyl diphosphate pH 7.5, 37°C Thermobifida fusca