EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.186 | preparation of a truncated already glucosylated His-tag fusion protein in Escherichia coli ER2566, expression of the truncated non-glucosylated His-tag fusion protein in Escherichia coli CGSC 4997 | Oryctolagus cuniculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.186 | 34000 | - |
non-glycosylated truncated protein, gel filtration of a 0.0005 mM solution | Oryctolagus cuniculus |
2.4.1.186 | 38000 | - |
1 * 38000, native enzyme, 50-70% of the activity of the dimer | Oryctolagus cuniculus |
2.4.1.186 | 38000 | - |
2 * 38000, native enzyme | Oryctolagus cuniculus |
2.4.1.186 | 59000 | - |
non-glycosylated truncated protein, gel filtration of a 0.02 mM solution | Oryctolagus cuniculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.186 | UDP-glucose + glycogenin | Oryctolagus cuniculus | essential for the formation of glycogen granules, binds a chain of 5-13 glucose molecules at a specific tyrosine residue by autoglycosylation, catalyzes two chemically different autoglucosylation reactions, the glucosylation of a tyrosine hydroxyl group and the formation of alpha-1,4 glucosidic linkages by subsequent glucosylations | UDP + glucosylated glycogenin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.186 | Oryctolagus cuniculus | P13280 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.4.1.186 | glycoprotein | autoglycosylation | Oryctolagus cuniculus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.186 | recombinant proteins | Oryctolagus cuniculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.186 | muscle | - |
Oryctolagus cuniculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.186 | UDP-glucose + glycogenin | - |
Oryctolagus cuniculus | UDP + glucosylated glycogenin | - |
? | |
2.4.1.186 | UDP-glucose + glycogenin | essential for the formation of glycogen granules, binds a chain of 5-13 glucose molecules at a specific tyrosine residue by autoglycosylation, catalyzes two chemically different autoglucosylation reactions, the glucosylation of a tyrosine hydroxyl group and the formation of alpha-1,4 glucosidic linkages by subsequent glucosylations | Oryctolagus cuniculus | UDP + glucosylated glycogenin | - |
? | |
2.4.1.186 | UDP-xylose + glycogenin | - |
Oryctolagus cuniculus | UDP + xylosylated glycogenin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.186 | dimer | 2 * 34000, non-glycosylated truncated protein, 0.02 mM enzyme solution | Oryctolagus cuniculus |
2.4.1.186 | dimer | 2 * 38000, native enzyme | Oryctolagus cuniculus |
2.4.1.186 | monomer | 1 * 34000, non-glycosylated truncated protein, 0.0005 mM enzyme solution | Oryctolagus cuniculus |
2.4.1.186 | monomer | 1 * 38000, native enzyme, 50-70% of the activity of the dimer | Oryctolagus cuniculus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.186 | glycogenin | - |
Oryctolagus cuniculus |