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Literature summary extracted from

  • De Vieira Santos, M.M.; SantAna, C.D.; Giglio, J.R.; da Silva, R.J.; Sampaio, S.V.; Soares, A.M.; Fecchio, D.
    Antitumoural effect of an L-amino acid oxidase isolated from Bothrops jararaca snake venom (2008), Basic Clin. Pharmacol. Toxicol., 102, 533-542.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.3.2 medicine significantly inhibits Ehrlich ascites tumour growth and induces an influx of polymorphonuclear cells, as well as spontaneous liberation of H2O2 from peritoneal macrophages. Later, LAAO-I induces mononuclear influx and peritoneal macrophage spreading. Animals treated with LAAO-I show higher survival time Bothrops jararaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 60000
-
 2 * 60000, SDS-PAGE Bothrops jararaca
1.4.3.2 125000
-
 gel filtration Bothrops jararaca

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Bothrops jararaca Q6TGQ9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 by gel filtration, affinity and hydrophobic interaction chromatography Bothrops jararaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
 Bothrops jararaca
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-isoleucine + H2O + O2 high substrate activity Bothrops jararaca 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-leucine + H2O + O2 high substrate activity, most specific substrate Bothrops jararaca 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-methionine + H2O + O2 high substrate activity Bothrops jararaca 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-phenylalanine + H2O + O2 high substrate activity, most specific substrate Bothrops jararaca phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 additional information L-serine, L-proline, L-glycine, L-threonine and L-cysteine are not oxidized Bothrops jararaca ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 homodimer 2 * 60000, SDS-PAGE Bothrops jararaca

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 LAAO-I
-
 Bothrops jararaca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.2 45
-
 after heating to 45°C, LAAO activity gradually decreases. After heating at 60°C and 70°C for 10 min., 85% and 100% of activity is lost, respectively. Freezing at -70°C for 24 h causes a 50% loss of enzyme activity. After lyophilization, about 15% only of activity is retained Bothrops jararaca

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.2 Bothrops jararaca
-
-
 5