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Literature summary extracted from

  • Arai, T.; Kino, K.
    A cyanophycin synthetase from Thermosynechococcus elongatus BP-1 catalyzes primer-independent cyanophycin synthesis (2008), Appl. Microbiol. Biotechnol., 81, 69-78.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.29 additional information no activation by DTT Thermosynechococcus vestitus
6.3.2.30 additional information no activation by DTT Thermosynechococcus vestitus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.29 gene cphA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional overexpression of His-tagged Tlr2170 in Escherichia coli BL21(DE3) Thermosynechococcus vestitus
6.3.2.30 gene cphA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional overexpression of His-tagged Tlr2170 in Escherichia coli BL21(DE3) Thermosynechococcus vestitus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.29 KCl activates Thermosynechococcus vestitus
6.3.2.29 Mg2+ Mg2+ or Mn2+ are required Thermosynechococcus vestitus
6.3.2.29 Mn2+ Mg2+ or Mn2+ are required Thermosynechococcus vestitus
6.3.2.30 KCl activates Thermosynechococcus vestitus
6.3.2.30 Mg2+ Mg2+ or Mn2+ are required Thermosynechococcus vestitus
6.3.2.30 Mn2+ Mg2+ or Mn2+ are required Thermosynechococcus vestitus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.29 100000
-
 4 * 100000, SDS-PAGE Thermosynechococcus vestitus
6.3.2.29 400000
-
 the molecular mass of cyanophycin increases with increasing reaction temperature Thermosynechococcus vestitus
6.3.2.30 100000
-
 4 * 100000, SDS-PAGE Thermosynechococcus vestitus
6.3.2.30 400000
-
 the molecular mass of cyanophycin increases with increasing reaction temperature Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp Thermosynechococcus vestitus
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 additional information Thermosynechococcus vestitus CphA requires L-Asp, L-Arg, ATP, Mg2+, and low-molecular mass cyanophycin as a primer for cyanophycin synthesis and catalyzes the elongation of a low-molecular mass cyanophycin, overview ?
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Thermosynechococcus vestitus
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 additional information Thermosynechococcus vestitus CphA requires L-Asp, L-Arg, ATP, Mg2+, and low-molecular mass cyanophycin as a primer for cyanophycin synthesis and catalyzes the elongation of a low-molecular mass cyanophycin, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.29 Thermosynechococcus vestitus
-
 gene cphA
-
6.3.2.30 Thermosynechococcus vestitus
-
 gene cphA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.29 recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration Thermosynechococcus vestitus
6.3.2.30 recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration Thermosynechococcus vestitus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp
-
 Thermosynechococcus vestitus ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp primer and product analysis using recombinantly expressed Tlr2170 protein, overview Thermosynechococcus vestitus ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 additional information CphA requires L-Asp, L-Arg, ATP, Mg2+, and low-molecular mass cyanophycin as a primer for cyanophycin synthesis and catalyzes the elongation of a low-molecular mass cyanophycin, overview Thermosynechococcus vestitus ?
-
 ?
6.3.2.29 additional information the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29. Recombinant Tlr2170 protein catalyzes in vitro cyanophycin synthesis in the absence of a primer. The Tlr2170 protein shows strict substrate specificity toward L-Asp and L-Arg Thermosynechococcus vestitus ?
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 Thermosynechococcus vestitus ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg primer and product analysis using recombinantly expressed Tlr2170 protein, overview Thermosynechococcus vestitus ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 additional information CphA requires L-Asp, L-Arg, ATP, Mg2+, and low-molecular mass cyanophycin as a primer for cyanophycin synthesis and catalyzes the elongation of a low-molecular mass cyanophycin, overview Thermosynechococcus vestitus ?
-
 ?
6.3.2.30 additional information the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29. Recombinant Tlr2170 protein catalyzes in vitro cyanophycin synthesis in the absence of a primer. The Tlr2170 protein shows strict substrate specificity toward L-Asp and L-Arg Thermosynechococcus vestitus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.29 tetramer 4 * 100000, SDS-PAGE Thermosynechococcus vestitus
6.3.2.30 tetramer 4 * 100000, SDS-PAGE Thermosynechococcus vestitus

Synonyms

EC Number Synonyms Comment Organism
6.3.2.29 CphA
-
 Thermosynechococcus vestitus
6.3.2.29 cyanophycin synthetase
-
 Thermosynechococcus vestitus
6.3.2.29 More cf. EC 6.3.2.30 Thermosynechococcus vestitus
6.3.2.29 Tlr2170 protein
-
 Thermosynechococcus vestitus
6.3.2.30 CphA
-
 Thermosynechococcus vestitus
6.3.2.30 cyanophycin synthetase
-
 Thermosynechococcus vestitus
6.3.2.30 More cf. EC 6.3.2.29 Thermosynechococcus vestitus
6.3.2.30 Tlr2170 protein
-
 Thermosynechococcus vestitus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.29 30
-
 assay at Thermosynechococcus vestitus
6.3.2.30 30
-
 assay at Thermosynechococcus vestitus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.3.2.29 30 70
-
 Thermosynechococcus vestitus
6.3.2.30 30 70
-
 Thermosynechococcus vestitus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.29 50
-
 purified recombinant Tlr2170 protein retains 80% activity after a 60 min incubation at 50°C Thermosynechococcus vestitus
6.3.2.29 70
-
 purified recombinant Tlr2170, inactivation Thermosynechococcus vestitus
6.3.2.30 50
-
 purified recombinant Tlr2170 protein retains 80% activity after a 60 min incubation at 50°C Thermosynechococcus vestitus
6.3.2.30 70
-
 purified recombinant Tlr2170, inactivation Thermosynechococcus vestitus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.29 9
-
-
 Thermosynechococcus vestitus
6.3.2.30 9
-
-
 Thermosynechococcus vestitus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.29 ATP
-
 Thermosynechococcus vestitus
6.3.2.30 ATP
-
 Thermosynechococcus vestitus