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Literature summary extracted from
- Purification and biochemical characterization of a broad substrate specificity thermostable alkaline protease from Aspergillus nidulans (2008), Appl. Microbiol. Biotechnol., 78, 603-612.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.1 | Cu2+ | 10 mM Cu2+ reduces the esterase activity by 65% | Aspergillus nidulans |  |
| 3.1.1.1 | Fe2+ | inhibition at 10 mM | Aspergillus nidulans | |
| 3.1.1.1 | Fe3+ | inhibition at 10 mM | Aspergillus nidulans | |
| 3.1.1.1 | Hg2+ | inhibition at 1 and 10 mM | Aspergillus nidulans | |
| 3.1.1.1 | additional information | EDTA, Mg2+, Mn2+, Ca2+, and Ba2+ do not affect the esterase activity, the enzyme is not sensitive to the addition of 1 mM SDS | Aspergillus nidulans | |
| 3.1.1.1 | Phenylmethylsulphonyl fluoride | inhibits at 10 mM | Aspergillus nidulans | |
| 3.1.1.1 | Zn2+ | inhibition at 10 mM | Aspergillus nidulans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.1 | Co2+ | 1 mM Co2+ increases activity up to 156% | Aspergillus nidulans | |
| 3.1.1.1 | Fe2+ | up to 238% activation of the enzyme is observed in the presence of 1 mM Fe2+ | Aspergillus nidulans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 37000 | - |
SDS-PAGE | Aspergillus nidulans |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | Acetone | 30% (v/v) acetone decreases the activity by 40%, 50% acetone results in a severe reduction of activity | Aspergillus nidulans |
| 3.1.1.1 | dimethylsulfoxide | 30% (v/v) dimethylsulfoxide decreases the activity by 40%, 50% (v/v) dimethylsulfoxide results in a severe reduction of activity | Aspergillus nidulans |
| 3.1.1.1 | Ethanol | 30% (v/v) ethanol increases the esterase activity by 20% | Aspergillus nidulans |
| 3.1.1.1 | isopropyl ether | 15% (v/v) isopropyl ether increases the esterase activity by about 60%, 50% (v/v) isopropyl ether produces a 10% increase in esterase activity | Aspergillus nidulans |
| 3.1.1.1 | Methanol | 30% (v/v) methanol increases the esterase activity by 10% | Aspergillus nidulans |
| 3.1.1.1 | SDS | the denaturing effect of SDS is noticeable at 10 mM | Aspergillus nidulans |
| 3.1.1.1 | Spa-80 | decreases activity at 0.1% (v/v) and 1% (v/v) | Aspergillus nidulans |
| 3.1.1.1 | taurocholic acid | slightly increases activity at 0.1% (v/v) | Aspergillus nidulans |
| 3.1.1.1 | Triton X-100 | decreases activity at 0.1% (v/v) and 1% (v/v) | Aspergillus nidulans |
| 3.1.1.1 | Tween | 0.1% Tween-80 increases activity by 160%, in the presence of 1% detergent, Tween-80 increases activity by 126% | Aspergillus nidulans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.1 | Aspergillus nidulans | - |
- |
- |
| 3.1.1.1 | Aspergillus nidulans PW1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | Ultrogel ACA-54 gel filtration and HPLC chromatography | Aspergillus nidulans |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 0.113 | - |
crude extract, at 25°C, pH 7.2 | Aspergillus nidulans |
| 3.1.1.1 | 5.62 | - |
after 49.73fold purification, at 25°C, pH 7.2 | Aspergillus nidulans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.1 | 2-nitrophenyl laurate + H2O | - |
Aspergillus nidulans | 2-nitrophenol + laurate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl acetate + H2O | - |
Aspergillus nidulans | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl acetate + H2O | - |
Aspergillus nidulans PW1 | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl laurate + H2O | - |
Aspergillus nidulans | 4-nitrophenol + laurate | - |
? | |
| 3.1.1.1 | alpha-naphthyl acetate + H2O | - |
Aspergillus nidulans | alpha-naphthol + acetate | - |
? | |
| 3.1.1.1 | alpha-naphthyl acetate + H2O | - |
Aspergillus nidulans PW1 | alpha-naphthol + acetate | - |
? | |
| 3.1.1.1 | dicaprin + H2O | - |
Aspergillus nidulans | ? | - |
? | |
| 3.1.1.1 | diolein + H2O | - |
Aspergillus nidulans | ? | - |
? | |
| 3.1.1.1 | diolein + H2O | - |
Aspergillus nidulans PW1 | ? | - |
? | |
| 3.1.1.1 | monocaprin + H2O | - |
Aspergillus nidulans | glycerol + caproate | - |
? | |
| 3.1.1.1 | monoolein + H2O | - |
Aspergillus nidulans | glycerol + oleic acid | - |
? | |
| 3.1.1.1 | monoolein + H2O | - |
Aspergillus nidulans PW1 | glycerol + oleic acid | - |
? | |
| 3.1.1.1 | monopalmitoin + H2O | - |
Aspergillus nidulans | glycerol + palmitoleic acid | - |
? | |
| 3.1.1.1 | additional information | the purified esterase is able to hydrolyze synthetic substrates with acyl group chain lengths of C4, C12, and C14 and displays higher activity (85% higher) toward C4 chain-lengths | Aspergillus nidulans | ? | - |
? | |
| 3.1.1.1 | additional information | the purified esterase is able to hydrolyze synthetic substrates with acyl group chain lengths of C4, C12, and C14 and displays higher activity (85% higher) toward C4 chain-lengths | Aspergillus nidulans PW1 | ? | - |
? | |
| 3.1.1.1 | tributyrin + H2O | - |
Aspergillus nidulans | butanoate + glycerol | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 40 | - |
- |
Aspergillus nidulans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 30 | 60 | - |
Aspergillus nidulans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 30 | 80 | the enzyme retains 80% of its activity after incubation at 30°C to 70°C for 30 min and loses 50% of its activity after incubation for 15 min at 80°C, the enzyme is relatively stable below 60°C, while the inactivation rate increases dramatically above this temperature | Aspergillus nidulans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 8.5 | - |
- |
Aspergillus nidulans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 7 | 10 | - |
Aspergillus nidulans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 6 | 11 | the enzyme retains activity after incubation at pHs ranging from 8 to 11 for 12 h at 37°C and 6 to 8 for 24 h at 37°C | Aspergillus nidulans |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.1.1 | Aspergillus nidulans | isoelectric focusing | - |
4.5 |
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