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Literature summary extracted from

  • Kontkanen, H.; Westerholm-Parvinen, A.; Saloheimo, M.; Bailey, M.; Raettoe, M.; Mattila, I.; Mohsina, M.; Kalkkinen, N.; Nakari-Setaelae, T.; Buchert, J.
    A novel polyesterase from Coprinopsis cinereus hydrolysing cutin and suberin (2009), Appl. Environ. Microbiol., 75, 2148-2157.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.1.74 additional information can be exploited in treating agricultural, food, and forest raw materials as well as their processing by-products Coprinopsis cinerea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.74 cloned and expressed in Trichoderma reesei Coprinopsis cinerea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.74 6982
-
 N-terminal fragment, electrospray ionization Q-TOF mass spectrometric analysis Coprinopsis cinerea
3.1.1.74 12140
-
 C-terminal fragment, Ala(95) - His(204), electrospray ionization Q-TOF mass spectrometric analysis Coprinopsis cinerea
3.1.1.74 12270
-
 C-terminal fragment, Ala(95) - His(205), electrospray ionization Q-TOF mass spectrometric analysis Coprinopsis cinerea
3.1.1.74 18800
-
 around, electrospray ionization Q-TOF mass spectrometric analysis gives two masses 18726.0 Da and 18863.0 Da Coprinopsis cinerea
3.1.1.74 20000
-
 SDS-PAGE Coprinopsis cinerea

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.74 Coprinopsis cinerea B9U443
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.74 by immobilized metal affinity chromatography exploiting a C-terminal His-tag Coprinopsis cinerea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.74 cutin + H2O
-
 Coprinopsis cinerea cutin monomers
-
 ?
3.1.1.74 additional information CcCUT1 has higher activity on shorter (C2-C10) 12 fatty acid esters of p-nitrophenol than on longer ones and it also exhibited lipase activity. Microscopical analyses and determination of released hydrolysis products showed that the enzyme is able to depolymerize apple cutin and birch outer bark suberin Coprinopsis cinerea ?
-
 ?
3.1.1.74 p-nitrophenyl acetate + H2O concentration of substrate dispersion is 5 mM Coprinopsis cinerea p-nitrophenol + acetate
-
 ?
3.1.1.74 p-nitrophenyl butyrate + H2O concentration of substrate dispersion is 5 mM Coprinopsis cinerea p-nitrophenol + butyrate
-
 ?
3.1.1.74 p-nitrophenyl caprate + H2O concentration of substrate dispersion is 5 mM Coprinopsis cinerea p-nitrophenol + ?
-
 ?
3.1.1.74 p-nitrophenyl caproate + H2O concentration of substrate dispersion is 5 mM Coprinopsis cinerea p-nitrophenol + ?
-
 ?
3.1.1.74 p-nitrophenyl laurate + H2O concentration of substrate dispersion is 5 mM Coprinopsis cinerea p-nitrophenol + ?
-
 ?
3.1.1.74 p-nitrophenyl myristate + H2O concentration of substrate dispersion is 5 mM Coprinopsis cinerea p-nitrophenol + ?
-
 ?
3.1.1.74 p-nitrophenyl palmitate + H2O concentration of substrate dispersion is 5 mM Coprinopsis cinerea p-nitrophenol + ?
-
 ?
3.1.1.74 p-nitrophenyl propionate + H2O concentration of substrate dispersion is 5 mM Coprinopsis cinerea p-nitrophenol + ?
-
 ?
3.1.1.74 p-nitrophenyl stearate + H2O a lower concentration of p-nitrophenyl stearate (2.5 mM) is used due to its lower solubility Coprinopsis cinerea p-nitrophenol + ?
-
 ?
3.1.1.74 p-nitrophenyl valerate + H2O concentration of substrate dispersion is 5 mM Coprinopsis cinerea p-nitrophenol + pentanoate
-
 ?
3.1.1.74 suberin + H2O 9,10-epoxy-18-hydroxy 5 octadecanoic acid Coprinopsis cinerea ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.74 CcCUT1
-
 Coprinopsis cinerea
3.1.1.74 cutinase
-
 Coprinopsis cinerea
3.1.1.74 cutinolytic polyesterase
-
 Coprinopsis cinerea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.74 40
-
 activity assay at Coprinopsis cinerea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.74 50
-
 enzyme retains 80% of its activity after 20 h incubation at 50°C, but residual activity decreases sharply at 60°C Coprinopsis cinerea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.74 7
-
 and a second optimum at 8.0, but retains activity over a wide pH range Coprinopsis cinerea
3.1.1.74 8
-
 and a second optimum at 7.0, but retains activity over a wide pH range Coprinopsis cinerea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.74 additional information
-
 retains activity over a wide pH range Coprinopsis cinerea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.74 additional information
-
 retains activity over a wide pH range Coprinopsis cinerea