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Literature summary extracted from

  • Pokusaeva, K.; OConnell-Motherway, M.; Zomer, A.; Fitzgerald, G.F.; van Sinderen, D.
    Characterization of two novel alpha-glucosidases from Bifidobacterium breve UCC2003 (2009), Appl. Environ. Microbiol., 75, 1135-1143.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.10 His-tag Bifidobacterium breve

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.10 5.5
-
 isomaltotriose
-
 Bifidobacterium breve
3.2.1.10 7.1
-
 palatinose
-
 Bifidobacterium breve
3.2.1.10 8.3
-
 isomaltose
-
 Bifidobacterium breve
3.2.1.10 9
-
 isomaltose
-
 Bifidobacterium breve
3.2.1.10 10.4
-
 panose
-
 Bifidobacterium breve
3.2.1.10 15
-
 panose
-
 Bifidobacterium breve
3.2.1.10 16.7
-
 isomaltotriose
-
 Bifidobacterium breve
3.2.1.10 22.9
-
 Trehalulose
-
 Bifidobacterium breve
3.2.1.10 27.2
-
 palatinose
-
 Bifidobacterium breve
3.2.1.10 30.2
-
 Trehalulose
-
 Bifidobacterium breve
3.2.1.10 31.15
-
 trehalose
-
 Bifidobacterium breve
3.2.1.10 33.6
-
 trehalose
-
 Bifidobacterium breve

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.10 68220
-
 calculated Bifidobacterium breve
3.2.1.10 68320
-
 calculated Bifidobacterium breve
3.2.1.10 70000
-
 SDS-PAGE Bifidobacterium breve

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.10 Bifidobacterium breve C0JP82
-
-
3.2.1.10 Bifidobacterium breve C0JP83
-
-
3.2.1.10 Bifidobacterium breve UCC2003 C0JP82
-
-
3.2.1.10 Bifidobacterium breve UCC2003 C0JP83
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.10
-
 Bifidobacterium breve

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.10 0.4
-
 maltulose Bifidobacterium breve
3.2.1.10 0.4
-
 nigerose Bifidobacterium breve
3.2.1.10 0.5
-
 turanose Bifidobacterium breve
3.2.1.10 0.7
-
 nigerose Bifidobacterium breve
3.2.1.10 1.1
-
 maltulose Bifidobacterium breve
3.2.1.10 1.6
-
 trehalose Bifidobacterium breve
3.2.1.10 1.6
-
 turanose Bifidobacterium breve
3.2.1.10 2
-
 trehalose Bifidobacterium breve
3.2.1.10 3.2
-
 palatinose Bifidobacterium breve
3.2.1.10 4.1
-
 palatinose Bifidobacterium breve
3.2.1.10 7
-
 isomaltotriose Bifidobacterium breve
3.2.1.10 8.6
-
 isomaltotriose Bifidobacterium breve
3.2.1.10 10
-
 trehalulose Bifidobacterium breve
3.2.1.10 11.7
-
 trehalulose Bifidobacterium breve
3.2.1.10 14.1
-
 isomaltose Bifidobacterium breve
3.2.1.10 19.3
-
 panose Bifidobacterium breve
3.2.1.10 20.4
-
 isomaltose Bifidobacterium breve
3.2.1.10 23
-
 panose Bifidobacterium breve

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.10 isomaltose + H2O
-
 Bifidobacterium breve alpha-D-glucose + D-glucose
-
 ?
3.2.1.10 isomaltose + H2O
-
 Bifidobacterium breve UCC2003 alpha-D-glucose + D-glucose
-
 ?
3.2.1.10 isomaltotriose + H2O
-
 Bifidobacterium breve 3 alpha-D-glucose
-
 ?
3.2.1.10 isomaltotriose + H2O
-
 Bifidobacterium breve UCC2003 3 alpha-D-glucose
-
 ?
3.2.1.10 maltulose + H2O
-
 Bifidobacterium breve alpha-D-glucose + D-fructose
-
 ?
3.2.1.10 maltulose + H2O
-
 Bifidobacterium breve UCC2003 alpha-D-glucose + D-fructose
-
 ?
3.2.1.10 nigerose + H2O
-
 Bifidobacterium breve alpha-D-glucose + D-glucose
-
 ?
3.2.1.10 nigerose + H2O
-
 Bifidobacterium breve UCC2003 alpha-D-glucose + D-glucose
-
 ?
3.2.1.10 palatinose + H2O
-
 Bifidobacterium breve alpha-D-glucose + D-fructose
-
 ?
3.2.1.10 palatinose + H2O
-
 Bifidobacterium breve UCC2003 alpha-D-glucose + D-fructose
-
 ?
3.2.1.10 panose + H2O preference for the cleavage of the alpha-1,6 linkage Bifidobacterium breve alpha-D-glucose + maltose
-
 ?
3.2.1.10 trehalose + H2O
-
 Bifidobacterium breve 2 alpha-D-glucose
-
 ?
3.2.1.10 trehalulose + H2O
-
 Bifidobacterium breve alpha-D-glucose + beta-D-fructose
-
 ?
3.2.1.10 turanose + H2O
-
 Bifidobacterium breve alpha-D-glucose + beta-D-fructose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.10 alpha-glucosidase
-
 Bifidobacterium breve
3.2.1.10 alpha-glucosidase 2
-
 Bifidobacterium breve

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.10 30
-
-
 Bifidobacterium breve
3.2.1.10 37
-
-
 Bifidobacterium breve

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.10 5.8
-
 trehalose
-
 Bifidobacterium breve
3.2.1.10 7.3
-
 trehalose
-
 Bifidobacterium breve
3.2.1.10 12
-
 isomaltotriose
-
 Bifidobacterium breve
3.2.1.10 12.4
-
 palatinose
-
 Bifidobacterium breve
3.2.1.10 12.5
-
 isomaltotriose
-
 Bifidobacterium breve
3.2.1.10 13
-
 palatinose
-
 Bifidobacterium breve
3.2.1.10 28.7
-
 panose
-
 Bifidobacterium breve
3.2.1.10 30
-
 panose
-
 Bifidobacterium breve
3.2.1.10 30.8
-
 isomaltose
-
 Bifidobacterium breve
3.2.1.10 35.4
-
 Trehalulose
-
 Bifidobacterium breve
3.2.1.10 36.3
-
 isomaltose
-
 Bifidobacterium breve
3.2.1.10 38.7
-
 Trehalulose
-
 Bifidobacterium breve

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.10 5.5
-
-
 Bifidobacterium breve