Literature summary extracted from

Smagin, V.A.; Mardanov, A.V.; Bonch-Osmolovskaya, E.A.; Ravin, N.V.
Isolation and characteristics of new thermostable DNA ligase from archaea of the genus Thermococcus (2008), Appl. Biochem. Microbiol., 44, 473-477.

No PubMed abstract available

Application

EC Number	Application	Comment	Organism
6.5.1.1	molecular biology	LihTh1519 may be used for basic and applied researches in molecular biology and genetic engineering	Thermococcus sp.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
6.5.1.1	expressed in Escherichia coli strain DLT1270/pRARE	Thermococcus sp.

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
6.5.1.1	Mg2+	optimum concentration at 50 mM	Thermococcus sp.
6.5.1.1	Na+	optimum concentration at 100 mM	Thermococcus sp.

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
6.5.1.1	63000	-	SDS-PAGE	Thermococcus sp.

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.5.1.1	Thermococcus sp.	C0LJI8	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
6.5.1.1	Ni-NTA column chromatography	Thermococcus sp.

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.5.1.1	ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	LigTh1519 is capable of ligating the cohesive ends and single-strand breaks in double-stranded DNA with ATP as cofactor	Thermococcus sp.	AMP + diphosphate + (deoxyribonucleotide)m+n	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
6.5.1.1	DNA ligase	-	Thermococcus sp.
6.5.1.1	LigTh1519	-	Thermococcus sp.

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
6.5.1.1	65	70	-	Thermococcus sp.

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
6.5.1.1	50	75	the enzyme remains active at 50-75°C or higher	Thermococcus sp.

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
6.5.1.1	80	-	more than 50% Lig1519 activity is preserved after incubation of the enzyme at 80°C for 30 min	Thermococcus sp.

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5.1.1	7	10.5	-	Thermococcus sp.

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
6.5.1.1	ATP	-	Thermococcus sp.

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Release 2023.1 (January 2023)